响应面法优化以菊芋全粉为碳源的双歧杆菌增殖培养条件

Optimization of Culture Conditions for Bifidobacterial Enrichment with Whole Powder of Jerusalem artichoke Tubers as Carbon Source by Response Surface Analysis

以菊芋全粉、菊粉和葡萄糖作为培养基碳源,研究其对双歧杆菌生长情况的影响,并研究菊芋全粉的发酵性能,运用Design-Expert 8.0设计实验并以响应面分析的方法进行发酵条件优化。结果表明:菊芋全粉、菊粉作为单一碳源对双歧杆菌具有增殖作用,利用菊芋全粉对双歧杆菌的增殖作用要优于菊粉和葡萄糖,双歧杆菌的增殖数量分别为菊粉的5.3倍、葡萄糖的16.8倍,菊芋全粉作为双歧杆菌培养基的碳源的最优发酵条件为:菊芋全粉添加量21g/L、温度36℃、接种量10%。

The best carbon source for bifidobacterial growth was selected from inulin, glucose and whole powder of Jerusalem artichoke Tubers, and the bifidobacterial enrichment culture conditions based on the selected carbon source were optimized by experimental design using Design-Expert 8.0 and response surface analysis. Both inulin and glucose were good bifidobacterial growth promoters when used as sole carbon sources; however, whole powder of Jerusalem artichoke Tubers was more effective in promoting bifidobacterial growth, providing a 5.3-fold and 16.8-fold increase in bifidobacterial populations after enrichment culture compared to inulin and glucose, respectively. When whole powder of Jerusalem artichoke Tubers was used as the carbon source, the optimal culture conditions were 36 ℃, 21 g/L of carbon source concentration and 10% of inoculum concentration.