青钱柳多糖对3T3-L1前脂肪细胞增殖分化的影响

Effect of Cyclocarya paliurus (Batal.) Iljinskaja Polysaccharide on the Proliferation and Differentiation of 3T3-L1 Preadipocytes

为探讨青钱柳多糖对3T3-L1前脂肪细胞增殖分化的影响。以青钱柳多糖为研究对象,通过D301-R大孔吸附树脂和DEAE纤维素阴离子树脂分离纯化,得到青钱柳多糖组分(CPC),采用凝胶渗透色谱(GPC)和多角度极光散射(MALLS)法分析其分子质量;采用不同质量浓度的多糖作用于3T3-L1脂肪细胞,采用MTT法和油红O染色检测多糖对脂肪细胞增殖分化的影响,同时测定细胞葡萄糖的消耗和细胞外甘油含量。结果表明:CPC含有3个组分,其黏均分子质量(Mη)分别为50300、3951g/mol和1406g/mol;在一定的质量浓度范围内,CPC对前脂肪细胞的生长表现为增殖趋势,且呈现一定的量效关系;与对照组相比,青钱柳多糖能够降低3T3-L1脂肪细胞对葡萄糖的消耗,降低3T3-L1脂肪细胞细胞外甘油含量,其抑制率分别高达9.61%和30.8%。提示青钱柳多糖在一定质量浓度范围能够促进脂肪细胞的增殖,降低葡萄糖的消耗和细胞外甘油含量。

This study was conducted to investigate the effect of Cyclocarya paliurus （Batal.） Iljinskaja polysaccharide on the proliferation and differentiation of 3T3-L1 preadipocytes. The crude polysaccharide extract of C. paliurus was fractionated by using D301-R macroporous resin and DEAE-anion fiber resin, and a C. paliurus polysaccharide component （CPC） was obtained. Gel permeation chromatography （GPC） and multi-angle laser light scattering （MALLS） were then used to determine its molecular weight distribution. The proliferation and differentiation of 3T3-L1 preadipocytes treated with different CPC concentrations were measured by MTT and oil red O staining, respectively. At the same time the glucose consumption and extracellular glycerol content were determined. Results showed that CPC consisted of three different components and their viscosity-average molecular weight （Mη） was determined to be 50300, 3951 g/mol and 1406 g/mol, respectively. Within a certain concentration range, CPC affected the proliferation of preadipocytes in a concentration- dependent manner. Compared with the control group, CPC could reduce the glucose consumption and extracellular glycerin content of 3T3-L1 adipocytes with inhibition ratios of as high as 9.61% and 30.8% respectively. These observations reveal that the C. paliurus polysaccharide is capable of promoting the proliferation of adipocytes in certain concentration range and reducing glucose consumption and extracellular glycerin content.