甘蔗原生质体分化成根

Root Organogenesis from Sugarcane Protoplasts

用 MS 培养基,从甘蔗嫩叶或幼穗诱导愈伤组织,将愈伤组织放置 MS 液体培养基中悬浮培养,每3—5d 继代一次。MS 培养基附加2,4—D3mg/L,水解乳蛋白500mg/L,盐酸硫胺素1mg/L 以及椰子水5%。经4至5个月培养的悬浮培养物所分离的原生质体具有很强繁殖能力。原生质体植板于 K8P 琼脂糖培养基,持续细胞分裂和生长。原生质体衍生的无性系移到 MS 琼脂培养基,形成愈伤组织。9个糖蔗品种的原生质体形成这种愈伤组织,其中一个品种的愈伤组织在不含任何植物生长调节剂的 MS 培养基中分化出根来。

Cell suspensions were initiated from leaf or panicle callus and sub-cultured every 3 to 5 days by placing them in MS liquid medium containing 3 mg/L 2,4-D,50Omg/L lactalbumin hydrolysate,1 mg/L thiamine-HCl and 5% V/V Coconut water.After cultured for 4 to 5 months the suspension cultures had the most reproduciable yields of protoplasts.Protoplasts plating in K8P agarose medium were sustaining division and growth.Transferring the pro- toplast-derived cell colonies to MS agar medium resulted the formation of calli.9 commercial sugarcane cultivar protoplasts formed calli.One of them gave root organogenesis when transferred to MS medium without any plant growth regulators.