飞秒时间分辨荧光非共线光参量放大光谱技术

Femtosecond time-resolved fluorescence non-collinear optical parametric amplification spectroscopy

时间分辨荧光光谱技术是研究激发态弛豫、能量传递以及电荷转移等光化学过程的重要且直接的工具.飞秒时间分辨荧光非共线光参量放大光谱技术是一种新发展的具有高时间分辨率、宽探测带宽、高增益的时间分辨光谱技术.本文对该技术的基本原理与工作特性、系统配置、荧光收集和会聚、数据采集模式、时间分辨光谱数据处理等进行了系统阐述.最后简单介绍了飞秒时间分辨荧光非共线光参量放大光谱技术在物理、生物和化学领域的3个典型应用,并以此展现该技术的高时间分辨率、宽光谱探测及高增益优势.

Time-resolved fluorescence spectroscopy is a powerful and direct tool to study the photochemistry processes, such as excited state relaxation, energy transfer and electron transfer. Femtosecond time-resolved fluorescence non-collinear optical parametric amplification spectroscopy is a newly developed spectroscopy with high time resolution, broad detection spectrum band and high signal gain. In this paper, a system introduction for this spectroscopy, including basic principle and performance characteristics, system configurations, fluorescence collection and convergence, modes of data collection and time-resolved spectral data processing, is presented. At last, three typical applications in physics, biology and chemistry are provided respectively, through which the spectroscopy advantages, i.e., high time resolution, broad detection spectrum band and high signal gain, are further demonstrated.