大豆花叶病毒病和疫霉根腐病抗性的SSR标记辅助鉴定

SSR Identification of Soybean Line with Resistance to Both Soybean Mosaic Virus and Phytophthpra Root Rot

采用抗花叶病毒病品种东农93046、抗疫霉根腐病品种Conrad、耐疫霉根腐病品种合丰25和高产品种东农L-5两两杂交后再复交衍生的146个F2：5重组自交系，利用与大豆花叶病毒病抗性基因相关的SSR标记Satt114，与大豆疫霉根腐病抗性相关的分子标记Satt325、Satt343、Satt428、Satt005、Satt600、Satt611、Satt689、Satt579、Satt252、Satt274进行分子辅助鉴定，进而分别通过摩擦接种法和菌土法来验证分子辅助鉴定的准确性。结果表明：通过Satt114分子辅助鉴定共发现32个抗大豆花叶病毒病家系，经摩擦接种法表型验证其中28个家系表现为抗病，即分子辅助鉴定的准确性为87．5％。通过与大豆疫霉根腐病相关的10个SSR标记的分子辅助鉴定，共发现23个家系含有4～8个抗性位点，经菌土法表型验证其中含有4，5，6，7，8个抗病位点家系的病害损失率分别可达61．11％、47．08％、32．92％、26．11％、18．34％，即后代家系聚合越多的QTL则抗大豆疫霉根腐病能力越强。另外，本研究得到了4份既高抗大豆花叶病毒病又高抗大豆疫霉根腐病的新种质。

Soybean mosaic virus（SMV） and phytophthora root rot（PRR） are both worldwide soybean diseases, which seriously affect the yield and quality of soybean. Selecting resistance varieties is one of the most effective measures to control these two diseases. Traditional program for varieties with resistance to disease is time-consuming, laborious and inefficient. SSR marker and molecular-assisted breeding make effective selection of varieties with resistance to disease possible. A recombinant intercross line population including 146 F2：s line was derived from a cross between F1 （ Dongnong93046 × Hefeng25 ） × F1 （ Congrad ×DongnongL-5）. SSR marker Satt114, identified by Teng et al （ 2005 ）, and Satt325, Satt343, Satt428, Satt005, Satt600, Satt611 ,Satt689,Satt579,Satt252,Satt274 identified by Li et al（2010）were used to analyze these RI line with resistance to SMV and PRR. Moreover,phenotypic analysis for SMV and PRR was used to evaluate SSR identification. A total of 32 lines are resistance to SMV, 28 of which are verified through friction inoculation test. The accuracy of molecular assistedbreeding is 87.5%. A total of 23 lines have 4-8 resistance loci to PRR, loss rate of which are 61.11% ,47.08%, 32.92% ,26.11%, 18.34% , respectively. In this study ,four lines are highly resistant to both SMV and PRR.