广适性转bar基因大豆除草剂草丁膦筛选浓度的研究

Study on Screening Concentration of Wide Adaptability Herbicide-Resistant bar-Transgenic Soybean

草丁膦作为转基因筛选剂具有高效、快速、简便等优点。为了提高筛选转基因大豆阳性植株的效率，以6个常用的转基因受体大豆品种为材料，在温室和田间，采用叶片涂抹和整株喷施两种方法，通过受体品种叶片颜色变化和致死浓度确定了转基因后代材料适宜筛选浓度，其中适宜涂抹浓度为100～150mg·L^-1，适宜喷施浓度为700～750mg·L^-1。以转BAr基因T1代大豆植株进行验证，筛选浓度筛选准确率分别达到61．3％和73．9％。本研究为抗草丁膦转基因大豆后代材料的筛选提供了重要依据。

Glufosinate proves to be effective and convenient in screening transgenic soybean plants. To improve the screening efficiency of transgenic positive plants,the glufosinate screening concentrations were determined by two methods （daubing and spraying） in the greenhouse and field,with six common soybean transgenic receptor varieties as materials. The suitable glufosihate screening concentrations for daubing and spraying were 100-150 and 700-750mg·L^-1, respectively, when determined by leaves color changes and mortality rate of soybean plants. Then the availability was tested by T1 plants of soybean transgenic lines with bar gene,the accuracy rate was 61.3% and 73.9% ,respectively. The results would facilitate the screening of glufosinate-resistant transgenic soybean progenies.