不同处理方法对内生细菌TS-3在2种蔬菜体内定殖的影响

in vivo Colonization Effect of Different Treatment Methods on The Endophytic Bacteria TS-3 in Two Kinds of Vegetables

[目的]了解蔬菜源内生细菌TS-3菌株的生物学效应。[方法]采用涂抹叶片、菌悬液浸种和浇灌3种方法,将利福平(200μg/ml)标记的内生细菌TS-3接种到生菜和甜椒2种蔬菜中,其后测定定殖情况。[结果]采用4×105cfu/ml的菌悬液对生菜进行涂抹叶片接种,3 d后叶片中菌株数量达到高峰,根中第7天达高峰;在接种甜椒叶片后的第7天,茎和叶中的含菌量均达到最高峰,并稳定在104cfu/ml,而根系中含菌量无明显变化。采用浇灌接种生菜,TS-3菌株在3 d后达高峰,在甜椒茎、叶中第9天达高峰,而后该菌的定殖数量稳定在104cfu/ml。采用菌悬液浸种,TS-3菌株在生菜叶中的定殖量在1.1×104cfu/ml,根中的定殖量在1.5×104cfu/ml,而在甜椒中,其定殖量是根>茎>叶,但定殖量都在103cfu/ml数量级。[结论]鉴于TS-3菌株在2种蔬菜中都能定殖,且不同的接种方法对其定殖量无数量级上的影响,所以采用较简单的浇灌接种方法较好。

[ Objective ] The aim was to understand biological effect of endophytic bacteria TS-3. [ Method] In order to test the colonization, the labeled endophytic bacteria TS-3 of Rifampicin （200 μg/ml） was inoculated into lettuce and pepper with different treatment methods of smearing inoculation method, watering inoculated method and soaking bacterial suspension method. [ Result] After the 4 x l0s cfu/ml of bac- teria suspension was inoculated on the lettuce leaf by using smearing inoculation method, the number of strains which were isolated from the leaf reached the peak after three days, but the number of strains form roots reached peak after seven days. While using the same method to in- oculate bacteria in pepper, the numbers of strains form roots and leaf reached the peak simultaneously after seven days. After the strain TS-3 was inoculated by using watering inoculated method, the strain TS-3 of lettuce reached the peak at the fifth day, while the peak came at the ninth day in the stem and leaf of sweet pepper. With soaking bacterial suspension method, the colonization of strain TS-3 in lettuce leaf were 1.1 ×10^4 efu/ml and 1.5 ×10^4 efu/ml in roots, but the colonization in pepper was root 〉 stem 〉 leaf. [Conclusion] There was less effect between the three treatment methods, so when the TS-3 is inoculated in lettuce or pepper, using the watering inoculated method is the most e- conomical and practical.