寻氏肌蛤蛋白水解液制备锌蛋白盐的研究

Study on Peptided-zinc Chelate of Hydrolyzed Protein from Musculus senhousei

[目的]利用寻氏肌蛤蛋白水解液制备高生物利用率的锌蛋白盐。[方法]以寻氏肌蛤为原料,通过限制性酶水解制备蛋白水解液,与锌进行螯合,以螯合率为试验指标,通过单因素和L9(34)正交优化试验,研究了pH、温度、时间、配位比、不同锌源对螯合率的影响。[结果]试验表明,寻氏肌蛤蛋白水解液制备锌蛋白盐的螯合试验最佳条件为:pH 5,温度90℃,时间30 min,配位比为2∶1,ZnSO4为锌源;此条件下,锌的螯合率为67.51%。[结论]研究可为寻氏肌蛤的深加工利用以及锌蛋白盐的制备提供参考依据。

[ Objective ] To study on peptided-zinc chelate of hydrolyzod protein from Musculua senhousei. [ Method ] Musculus senlwusei pepti- ded-zinc was produced by hydrolyzod Musculus senhousei protein chelating with zinc. Taking the experiment target by the chelated rate, the effects of five independent variables （pH, temperature, time, peptide/Zn^2＋ ratio, different source of zinc） were studied through single element experi- ment and L9 （3^4） orthogona] experiment. [ Result] The optimum condition was obtained as follows： pH 5, 90 ℃, 30 min, peptide/Zn^2＋ ratio 2： 1, ZnSO4 as source of zinc. Then the chelation of zinc was 67.51%. [ Conclusion] The study can provide reference basis for deeply processing of Musculus senhousei and preparation of peptided-zinc.