银耳多糖的提取工艺研究

Study on the Extraction Technology of Polysaccharide from Tremella fuciformis

[目的]研究热水浸提法提取银耳多糖的最佳工艺条件。[方法]采用苯酚-硫酸法测定银耳总多糖的含量,以提取温度、提取时间、提取次数、溶剂用量为4个影响因素,每个因素分别设计3个水平进行正交试验,优化银耳多糖的提取工艺。[结果]试验表明,热水浸提法提取银耳多糖的最佳工艺为40倍量水,90℃提取3 h,共提取2次,此条件下多糖得率最高。[结论]研究可为银耳多糖的开发应用提供参考依据。

[ Objective ] To study the optimum technique conditions of polysaccharide from Tremella fuciformis by hot water extraction method. [ Method] Total polysaccharide content was determined by phenol-sulfuric acid method. Four influencing factors of extraction temperature, ex- traction time, extraction times, solvent dosage were selected to design three levels orthogonal experiment. [ Result ] The optimal conditions were water （W/V） of 1： 40, extraction at 90 ℃ for 3 h, 2 times repeated. [ Conclusion] The study can provide reference basis for develop- ment and utilization of Tremella polysaccharides.