柽柳谷胱甘肽转移酶基因(ThGSTZ1)转入酵母的抗逆能力分析

Stress Tolerance of a Glutathione S- transferase Gene from Tamarix hispida(ThGSTZ1) in Saccharomyces cerevisiae

[目的]研究盐、重金属、低温和高温胁迫下,柽柳GST基因(命名为ThGSTZ1)的抗性作用。[方法]将ThGSTZ1基因构建到酵母表达载体pYES2中,转入酿酒酵母(Saccharomyces cerevisiae)获得重组型酵母,同时以转化空载体pYES2的重组酵母作为阴性对照;对2种重组酵母分别进行LiCl、NaCl、KCl、CdCl2、ZnCl2、MgCl2、-20和53℃胁迫处理,比较2种酵母的成活率。[结果]ThGSTZ1能有效的提高酵母的抗盐碱及极端温度的能力,表明ThGSTZ1可能参与柽柳的逆境胁迫应答过程。[结论]GST基因具有参与多种胁迫响应和调节的功能。

[ Objective ] To study the stress tolerance of a glutathione S - transferase gene from T. hispida （ ThGSTZ1 ） under the stress of salt, heavy metal ,low temperature and high temperature. [ Method] The recombinant plasmid designated as pYES2-ThGSTZ1 was constructed by inser- ting the ThGSTZ1 gene into the yeast expression vector pYES2. The plasmid pYES2-ThGSTZI was transformed into the yeast Saccharomyces cerevi- siae, and the yeast transformed with emptypYES2 was used as a negative control. ThepYES2-ThGSTZ1 andpYES2 recombinant yeast were respec- tively treated with LiC1,NaC1,KC1,CdCI2 ,ZnC12, MgCl2 ; -20 and 53 ℃ the survival rate of two yeasts were compared. [ Result ] The results showed that the ThGSTZ1 was tolerant to all these stresses, suggesting that ThGSTZ1 may play an important role in stress tolerance of T. hispida. [ Conclusion] GST participates in several stress response and regulation.