肌肽对C57小鼠放射性肺损伤保护作用的机理研究

The Protected Effect of Carnocine on Radiation-induced Lung Injury in C57 Mice

[目的]观察肌肽(carnosine)对C57BL小鼠放射性肺损伤(RILI)的防护效果,并探讨其机制。[方法]将120只C57BL雌性小鼠随机分为对照组、给药组、辐照组和辐照+给药组,治疗后检测血清转化生长因子β1(TGF-β1)、丙二醛(MDA),以及部分肺组织羟脯氨酸(HYP)水平。[结果]除辐照后第7d血清中MDA水平各处理组间无明显差异(P>0.05),其余不同组间各时间段TGF-β1、MDA以及肺组织HYP水平差别均具有统计学意义(P<0.05或<0.01)。给药组较对照组未出现明显副作用。[结论]肌肽能降低血清、肺组织中TGF-β1、MDA、HYP含量,并具有减轻放射性肺损伤的作用,且几乎无副作用,在防治放射性肺损伤中显示出良好前景。

[Purpose] To investigate the intervention of carnosine on radiation-induced lung injury in C57 mice.[Methods] A total of 120 mice C57/BL（female） were divided into 4 groups：control group,carnosine group,irradiation group and irradiation plus carnosine group.After treatment,serum transforming growth factor-β1（TGF-β1）,malondialdehyde（MDA） were detected by ELISA assay and thiobarbituric acid colorimetric method respectively,while hydroxyproline（HYP） in parts of lung tissues was detected by spectrophotography.[Results] After irradiation for 7d,MDA levels of serum had no significant difference in the 4 groups（P0.05）.However,TGF-β1 levels of serum,MDA levels of serum and HYP levels of lung tissue had significant difference at different time periods（P0.05 or P0.01）.[Conclusions] Carnosine is able to reduce the level of TGF-β1,MDA,HYP and improve radiation-induced lung injury in mice.Carnosine is less toxicity and shows a good prospect in the treatment for radiation-induced lung injury.