方格星虫多糖抗菌和抗氧化活性研究

Studies on the Antimicrobial and Antioxidant Activities of Polysaccharide fromSipunculus nudus

通过比较水提和碱提方格星虫粗多糖的抗菌和抗氧化活性,探讨这两种方法在方格星虫粗多糖提取中的优劣。结果,水提粗多糖对副溶血弧菌(Vibrio parahemolyticus)不敏感,对枯草芽孢杆菌(Bacillus subtilis)中度敏感,对其它细菌均为低敏感;碱提粗多糖对大肠杆菌(Escherichia coli)、金黄色葡萄球菌(Staphylococcus aures)和鳗弧菌(Vibrio anguillarum)低敏感,对白葡萄球菌(Staphylococcus cremoris)和副溶血弧菌中度敏感,对枯草芽孢杆菌和藤黄八叠球菌(Sarcina lutea)高度敏感。表明,碱提粗多糖的抗菌活性较水提粗多糖强。两种方法提取的方格星虫粗多糖均有一定的抗氧化性。水提和碱提多糖浓度均在800μg·mL-1时对羟自由基的清除能力最大,清除率分别为40.32%和39.86%;两种多糖浓度分别在800μg·mL-1和600μg·mL-1时对超氧自由基的抑制作用最大,抑制率分别为9.91%和5.86%。表明,水提方格星虫粗多糖的抗氧化性强于碱提粗多糖。

The antimicrobial and antioxidant activities of crude polysaccharide from Sipunculus nudus were studied by two extraction methods,water extraction and alkali extraction.The results show that the crude polysaccharide extracted by water is insensitive to Vibrio parahemolyticus,medium sensitive to Bacillus subtilis,and low sensitive to other bacteria,while that extracted by alkali is low sensitive to Escherichia coli,Staphylococcus aures and Vibrio anguillarum,medium sensitive to Staphy lococcus cremoris and Vibrio parahemolyticus,and hypersensitive to Bacillus subtilis and Sarcina lutea.The antimicrobial activity of alkali-extracted polysaccharide is higher than that of water-extracted polysaccharide.Both of the crude polysaccharides from S.nudus extracted by the two methods have some effects on antioxidant activities.The scavenging ability to hydroxyl radical（· OH） reaches the maximum when the concentrations of the two polysaccharide extracts are 800μg · mL-1,and the clearance rates of water-extracted polysaccharide and alkali-extracted polysaccharide are 40.32% and 39.86%,respectively.The inhibition ability to superoxide anion（O2-）reaches the maximum when the water-extracted polysaccharide concentration is 800μg · mL-1,while the alkali-extracted polysaccharide concentration is 600μg · mL-1.Their inhibition rates are 9.91% and 5.86%.In short,antioxidant activity of water-extracted polysaccharide is higher than that of alkali-extracted polysaccharide.