共表达HIV-1CN与IL-2基因产物免疫鼠T淋巴细胞亚群的研究

Experimental study on splenic T cell subsets amount in immunized mice with gene vaccine of HIV-1 CN gag gp120 and IL-2 gene

目的 :研究细胞因子在机体免疫应答过程中的免疫佐剂效应。方法 :以表达中国流行株HIV lgag gp12 0基因的核酸疫苗质粒 pcDNAGP及共表达中国流行株HIV lgag gp12 0基因与Ⅱ 2基因的核酸疫苗质粒 pGPH 2免疫Balb/c鼠 ,用流式细胞仪测定 10 0 0 0个免疫鼠脾淋巴细胞中CD4+,CD8T细胞数及CD4+/CD8+比值。结果 :pGIL 2与pcDNAGP比较 ,pGIL 2免疫鼠CD4 和CD8T细胞数明显提高。结论 :在机体的免疫应答过程中 ,细胞因子IL

Objective:To investigate whether pGPIL 2 could play and adjuvant role in immune response.Methods:Vaccine plasmids of pGPIL 2 were packed in liposome and transfeded into BHK 21 cells. The expression of HIV 1 fusion protein in the cells was determined by ELISA.The splenocytes from pGPIL 2 immunized mice were examined.Results:The number of CD4 +T cells and CD8 +T cells from splenic lymphocytes significantly increased in pGPIL 2 immunized mice.Conclusion:pGPIL 2 could play an adjuvant role in inmmune response.