摘要
分别以总皂苷、二醇型或三醇型皂苷、8种单体皂苷的含量为指标,对不同产地、不同年生人参样品进行质量评价,并分析其差异。建立可同时测定Rg1、Re、Rf、Rb1、Rb2、Rb3、Rc、Rd的高效液相色谱法(HPLC),运用该法对人参单体皂苷含量进行测定;总皂苷含量采用香草醛—硫酸法测定。结果表明:以总皂苷含量为评价指标,抚松地区人参品质最佳;以8种单体皂苷含量为评价指标,长白、汪清地区较优。其次,采用同一评价指标时,如样品为四年生,珲春地区皂苷含量较低;如样品为五年生,以抚松和长白地区品质较差。人参品质评价结果与测试指标、取样年龄等密切相关,对人参原料和产品的质量控制应建立更加细致稳定的评价系统。
With total saponin, glycol or triol type saponins and eight kinds of monomer ginsenoside con- tent as indexes, the quality of different years of ginseng samples in different production areas was evaluat- ed, and the difference was analyzed. A HPLC method for determining Rgl, Re, Rf, Rb1, Rb2, Rb3, Re and Rd (HPLC) was established, and the method was applied to determine the content of ginseng saponins ; the content of total saponin was determined with vanillin sulfuric acid method. The results show that for total saponin of Panax ginseng, ginseng samples in Fusong area are the best; for the evaluation index of eight kinds of monomer ginsenoside content, ginseng samples in Changbai and Wangqing areas are better. For the same index, if the samples are 4 years old, saponin content in Hunchun area is low; if the samples are 5 years old, the quality in Fusong and Changhai area is poor. The evaluation results are closely related to the testing index and the sampling age, and a more stable evaluation system should be established to control the quality of ginseng raw materials and products.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2013年第6期673-678,共6页
Journal of Jilin Agricultural University
基金
国家科技重大专项(2011ZX09401-305-10-03)
国家自然科学基金项目(31270391)
国家科技支撑计划项目(2011BAI03B01-02)
关键词
人参
单体皂苷
总皂苷
HPLC
质量评价
产地
吉林省
ginseng
monomer ginsenoside
total saponin
HPLC
quality evaluation
place of origin
Jilin province