茶条槭叶乙酸乙酯部位化学成分研究

Studies on Chemical Constituents from Ethyl Acetate Extracts of Acer ginnala Maxim. Leaves

对茶条槭(Acer ginnala Maxim.)叶的乙酸乙酯部位化学成分进行了系统研究。采用硅胶柱色谱法、半制备高效液相色谱法以及Sephadex LH-20凝胶柱色谱法等方法进行分离纯化,通过质谱、核磁共振谱等光谱数据鉴定化合物结构。结果分离得到14个化合物,分别鉴定为槲皮素-3-O-α-L-(3″-没食子酰基)-鼠李糖苷(1)、5,7,2′,3′,4′-五羟基黄酮(2)、山柰酚(3)、山柰酚-3-O-β-D-吡喃半乳糖苷(4)、槲皮素-3-O-β-D-来苏糖苷(5)、槲皮素(6)、槲皮素-3-O-β-D-吡喃半乳糖苷(7)、槲皮素-3-O-(2″-没食子酰基)-α-L-阿拉伯吡喃糖苷(8)、槲皮素-3-O-a-(2″-没食子酰基)-鼠李糖苷(9)、山柰酚-3-O-a-(2″-没食子酰基)-鼠李糖苷(10)、槭单宁(11)、没食子酸(12)、没食子酸甲酯(13)、β-谷甾醇(14)。其中化合物1,2,5,9,10为本属中首次分离得到,化合物4和8为茶条槭中首次分离得到。

To investigate the chemical constituents from ethyl acetate extracts of the leaves of Acer gin- nala Maxim, solvent extraction, Silica gel and Sephadex LH-20 column chromatography were used for isolation and their structures were identified by means of spectroscopic and chemical data. Fourteen com- pounds were isolated and identified as quercetin-3-O-a-L-（ 3＂-galloyl）-rhamnoside （ 1 ）, 5,7,2＇, 3＇, 4＇- pentahydroxy flavone （2）, kaempferol （ 3 ）, kaempferol-3-O-^-D-galactopyranoside （4）, quercetin-3-O-t3- D-lyxoside （ 5 ）, quercetin （6）, quercetin-3-O-^-D-galactopyranoside （ 7 ）, quercetin-3-O-a-L-（ 2＂-gal- loyt） -arabinopyranosid （ 8 ）, quercetin-3-O-a-L-（ 2＂-galloyl ）-rhamnoside （ 9 ）, kaempferol-3-O-a-L-（ 2＂- galloyl）-rhamnoside（10）, gallic acid （ 11 ）, methyl gallate （12）, acertannin （ 13 ） and ^-sitosterol （14）. Compounds 4 and 8 are isolated from the plants in genus Acer for the first time and compounds 1, 2, 5, 9 and 10 are isolated from the plants Acer ginnala Maxim. for the first time.