硫酸镁对急性肺动脉高压实验室模型的血流动力学影响

EFFECT OF MAGNESIUM-SULFATE ON BLOOD FLOW DYNAMICS IN ACUTE PULMONARY HYPERTENSION MODEL

目的探讨硫酸镁(MgSO_4)对实验室急性肺动脉高压模型的影响机制。方法 30只杂种犬,随机分为5组,空白组(control)、肺动脉高压(pulmonary hypertension,PH)组以及MgSO_4 7.0、MgSO_4 8.0和MgSO_4 9.0组,其中Control组不给于任何处理,PH组为急性肺动脉高压(acute pulmonary hypertension,APH),其余MgSO_4 7.0、MgSO_4 8.0及MgSO_4 9.0组造成APH稳定30min后,分别静脉给予MgSO_4并维持血MgSO_4浓度为7.0mmol/L、8.0mmol/L及9.0mmol/L。肺动脉、股动脉插管检测平均肺动脉压(mean pulmonary artery pressure,MPAP)、体循环平均动脉压(mean arterial pressure,MAP),静脉取血检测血浆APH血浆内皮素1(endothelin-1,ET-1)、血栓素(thromboxane A_2,TXA_2)以及6-酮前列腺素(6-keto-PGFla)。结果①PH组MPAP较Control组显著升高,MgSO_4 7.0、MgSO_4 8.0及MgSO_4 9.0组MPAP均较PH组显著下降,其中MgSO_4 9.0下降最明显;②PH组MAP较Control组显著降低,其余3组MAP较PH组显著升高,其中MgSO_4 9.0升高最明显。③PH组ET-1、TXA_2及6-ketoPGFla较Control组显著升高;MgSO_4 7.0、MgSO_4 8.0及MgSO_4 9.0组ET-1、TXA_2较PH组显著下降,而6-keto-PGFla显著升高,其中MgSO_4 9.0组ET-1、TXA_2及6-keto-PGFla较PH组变化最明显。结论 MgSO_4可显著降低肺动脉高压,改善血流动力学紊乱,这与其抑制缩血管因子并促进舒血管因子释放有关。

Objective To investigate the effect of hypertension. Methods Thirty Mongrel dogs were randomly magnesium sulfate for acute pulmonary divided into five groups： control group, pulmonary hypertension （PH） group, MgSO47.0, MgSO48.0, and MgSO49.0 group. The control group was not given any treatment, and the PH group was operated to induce acute pulmonary hypertension; the remaining three groups were induced for acute pulmonary hypertension and after 30 min magnesium sulfate were given through intravenous infusion to maintain the concentration of 7.0 mm/L, 8.0 mm/L and 9.0 ram/L, respectively. The mean pulmonary artery pressure （MPAP） and the circulation mean arterial pressure （MAP） were detected, and endothelin-1 （ ET-1 ）, thromboxane A2 （ TXA2 ） and 6-ketoPGFla were measured by immuoflorence method for each group. Results （①The MPAP in PH group were significantly higher than those in control group ; however MPAP in MgSOJ. 0, MgSO48.0 and MgS049.0 were MAP significantly lower, compared with that in PH group, and the lowest level in MgS049.0 group. ②The in PH group was significantly lower than that in control group; however MAP in MgS047. 0, MgSQ8.0 and MgSQ9.0 were significantly higher, compared with that in PH group, and the highest level in MgSO49.0 group. ③ET-1, TXA2 and 6-keto-PGFla in PH group were significantly higher than those in control group. However, ET-1, TXA2 most significantly decreaseds, 6-keto-PGFla increased in MgSO4 9. 0 group compared with those in PH group. Conclusion Magnesium sulfate significantly reduced acute pulmonary hypertension through improvement of circulation disorders by decrement of ET-1 and TXA2 and also increment of prostacyclin（ PGI2 ）.