长期大脑中动脉闭塞后远隔部位微管相关蛋白和胶质纤维酸性蛋白变化

Experimental research of MAP－ 2 and GFAP changes of distal areas after the long- term middle cerebral artery occlusion

目的探讨长期大脑中动脉闭塞 (MCAO)后丘脑、黑质网状部 (sNr)微管相关蛋白 (MAP－ 2)以及胶质纤维酸性蛋白 (GFAP)的变化。方法 35只 SD大鼠分为 7组，即正常组、伪手术组 2周组、伪手术 1个月组、 MCAO后 2周组、 1个月组、 3个月组和 6个月。每组 5只， Tamura法制作动物模型。脑切片分别用鼠脑 MAP－ 2、 GFAP抗体， ABC法染色。结果缺血后 1个月 MAP－ 2减少， GFAP增加， 6个月后丝极消失。结论神经细胞 MAP－ 2减少和胶质细胞 GFAP增加是长期 MCAO后丘脑、 sNr萎缩的基础。

Objective To investigate the changes of MAP－ 2 and GFAP of the distal areas after long- term the middle cerebral artery occlusion(MCAO).Method 35 SD rats were divided into 7 groups,that is normal group;false operating 2 week group;false operating 1 month groups;2 week group,1 month group,3 month group and 6 month group after MCAO.Each group was 5 rats.Ischemic modles were done by Tamura method.The brain stained with MAP－ 2 and GFAP by ABC method to research the changes of areas of the thamus,the areas and the length of the substantia niga reticulum(sNr).The counts of neurons and glicyts of the sNr.Result The areas of the thamus and sNr were increased.MAP－ 2 stains were lessen and GAFP increased after 6 month MCAO.Conclusion Neurons reduction and gliocytes increase are the base of the atrophy of thamus and sNr afer long- term MACO.