酸性电解水冰理化指标变化对副溶血性弧菌杀菌效果的影响

Effect of Physicochemical Properties of Electrolyzed Water Ice on Inactivation of Vibrio parahaemolyticus During Storage

为探究不同时间下电解水冰的理化特性变化对杀菌效果的影响,作者将5种不同质量浓度电解液（NaCl质量浓度为0.75、1、1.25、1.5、1.75 g/L）制备的电解水于-36℃下制成电解水冰,分别于0、2、4、6、8、10h测定其pH值、氧化还原电位（ORP）及有效氯浓度（ACC）,并研究了相应保存时间下电解水冰对副溶血性弧菌的杀灭能力 结果表明：0～6 h内,不同质量浓度间电解水冰的pH值变化不显著;而6h后,NaCl质量浓度≤1g/L制备的电解水冰,其pH变化速率小于NaCl质量浓度＞1 g/L的冰.不同浓度电解水冰的ORP、ACC随时间的增加而减少,但ORP变化几乎遵循同一趋势;相比NaCl质量浓度≤1.5 g/L制备的电解水冰,NaCl质量浓度＞1.5 g/L的ACC损失率较大.0～10 h内,NaCl质量浓度为1.5g/L及1.25 g/L的电解水冰对副溶血性弧菌的杀菌量差值最大,与其它所有相邻浓度间杀菌量差值相比,最高比值可达117.6倍.根据相关性分析可得,电解水冰的pH、ORP、ACC对杀菌量的相关系数r分别为0.831、0.787和0.944.

Electrolyzed water ice is a relatively new preservation and cold sterilization technology developed in recent years.To study the changes in physicochemical properties of electrolyzed water ice during storage and their effect on inactivation of Vibrio parahaemolyticus,five types of electrolyzed water ice prepared from different concentration of sodium chloride solution （0.75,1,1.25,1.5,1.75 g/L） and were frozen at-36 ℃.And a set of physicochemical properties,including pH values,oxidation reduction potential （ORP） and available chlorine concentration （ACC）,were measured on storage hours （0-10 h）,and the bactericidal efficiency on Vibrio parahaemolyticus by electrolyzed water ice was studied during storage.The results showed that pH value did not have significant difference （p ＞0.05） from 0 to 6h,while after 6 h the rate of pH change of electrolyzed water ice prepared from NaCl ≤1 g/L was less than that of NaCl concentration ＞ 1 g/L.ORP and ACC decreased gradually,and the ORP changes almost follow the same trend.However,the ACC loss rate of NaCl＞1.5 g/L was higher than that of NaCl ≤ 1.5 g/L.Compared sterilization with all other adjacent concentration difference,the difference of the bactericidal efficiency on Vibrio parahaemolyticus by the electrolyzed water ice of NaCl concentration 1.5 g/L and 1.25 g/L,the highest ratio was to 117.6 times.According to the correlation analysis,the respective correlation coefficients of pH,ORP,ACC on the bactericidal capacity were 0.831,0.787 and 0.944.