链霉菌HMJ-7的鉴定及其抗菌物质稳定性分析

Identifing of Streptomyce HMJ-7 Producing Antibacterial Substance and Analysis of the Antibacterial Substance Stability

为获得新型抗菌活性物质并应用于食品防腐,用土壤溶液悬浮法从江苏徐州奎河边上的土壤样品中分离到了一株链霉菌菌株,命名为HMJ-7。采用打孔法验证其产生的抗菌物质的抗菌能力,结果表明:该菌株对大肠杆菌、金黄色葡萄球菌、藤黄微球菌、蜡样芽孢杆菌等细菌以及酵母菌都有良好的抑制作用,抑菌圈直径都在2 cm以上。对黑曲霉、黄曲霉、米曲霉等霉菌没有抑菌活性;通过对HMJ-7菌株形态特征、培养特性、生理生化性状以及16S rDNA序列分析,初步将该菌株鉴定为链霉菌属灰红紫类群灰红链霉菌(Streptomyces griseoruber);采用不同的温度、pH、有机溶剂、紫外线条件处理该菌株产生的抑菌活性物质,并分别通过打孔法测定其对蜡样芽孢杆菌的抑制活性。结果显示,这些条件对抑制活性没有明显影响,说明该抗菌物质比较稳定。上述结果表明,HMJ-7菌株有较大的利用潜力,也为进一步分离纯化其抑菌物质提供了依据。

For obtaining new antibiotics and applying it to food preservation,A Streptomyce producing high antibacterial substance was screened from a sample obtained from Xuzhou kui river of Jiangsu.This strain was identified through it＇s morphological,physiological and biochemical properties.16S rDNA sequence analysis of HMJ-7 showed this strain was closely to Streptomyces griseoruber to 99％,So,it was identified as Griseorubro violaceus.The antibacterial experiment result showed that the antibacterial substance can obviously inhibit the growth of E.coli,Staphyloccocus aureus Rosenbach,Micrococcus luteus,Bacillus cereus and yeast.But can＇t inhibit the growth of Aspergillus niger,Aspergillus flavus,Aspergillus oryzae and other fungi.The following experiment result indicated that the antibacterial substance produced by this strain was stable at differen temperature,pH,ultraviolet light and organic solvent condition.