摘要
在有染色体规模扩大方法的联合，最新发达的下一代的定序的平台提供一个强大的工具从一个单个房间学习 genomics。这微型评论包括干细胞，癌症生物学和繁殖的药在生物医学的研究的几个区域总结单个房间 genomics 和他们的应用程序的技术。特别地，它在单个房间 exome 定序， RNA-seq，并且染色体定序加亮最近的进展。这些强大的技术的应用程序将在单个房间的水平在基因抄写和染色体组织的基本原则上打开新灯并且在多细胞的有机体改进我们细胞的异质和差异的理解。
The newly developed next-generation sequencing platforms, in combination with gcnome-scale amplification methods, provide a powerful tool to study genomics from a single cell. This mini-review summarizes the technologies of single cell genomics and their applications in several areas of biomedical research including stem cells, cancer biology and reproductive medicine. Particularly, it highlights recent advances in single cell exome sequencing, RNA-seq, and genome sequencing. The application of these powerful techniques will shed new light on the fundamental principles of gene transcription and genome organization at single-cell level and improve our understanding of cellular heterogeneity and diversity in multicellular organisms
基金
This work was supported by ＂973＂ Programs （Nos. 2012CB966300, 2011CB966204 and 2011CB965102） from the Ministry of Science and Technology in China, the International Science and Technology Cooperation Program of China （No. 2011DFB30010）, the National Natural Science Foundation of China （Grant Nos. 81271258, 31301184）, and the Natural Science Foundation of Jiangsu Province of China （No. DK2011321）.
关键词
基因组学
单细胞
基因组测序
医学技术
基因组结构
扩增方法
生物医学
生物细胞
single-cell genomics, next-generation sequencing, RNA-seq, single-nucleotide variation, copy-number variation, DNA methylation
