顺铂预处理对CIK杀伤非霍奇金淋巴瘤细胞的影响

Cytotoxic effect of preconditioning with Cisplatin on CIK against non Hodgkin lymphoma cells

目的探讨顺铂(DDP)预处理对细胞因子诱导的杀伤细胞(CIK)杀伤非霍奇金淋巴瘤细胞的影响。方法体外培养人类NHL细胞株Namalwa(Burkitt淋巴瘤细胞株)、SU—DHL-4(弥漫大B淋巴瘤细胞株)及CIK细胞,采用CFSE/PI双标法检测DDP预处理不同浓度、不同时间,CIK对非霍奇金淋巴瘤细胞的杀伤活性影响。应用SPSS 19.0统计学软件对数据进行统计学分析。结果体外诱导获得CIK细胞,随着DDP预处理浓度的增加,CIK细胞杀伤淋巴瘤细胞的活性随之增强,随着DDP预处理时间的延长,CIK细胞杀伤淋巴瘤细胞的活性亦增强。结论 DDP预处理淋巴瘤细胞后,CIK杀伤淋巴瘤细胞活性增强。

Objective To study the cytotoxic effect of Cisplatin pretreatment on cytokine induced killer （CIK） cells against non Hodgkin lymphoma cells.Methods Human NHL cell lines Namalwa （Burkitt lymphoma cell line）,SU-DHL-4 （diffuse large B lymphoma cell line） and CIK cells were cultivated in vitro,CFSE/PI double labeling method was used to detect the cytotoxic effect of CIK after DDP pretreatment non-Hodgkin lymphoma cells at different concentration,different time.SPSS 19.0 software was used for statistical analysis.Results The CIK cells were cultured.With the increase of DDP precondition concentration,the cytotoxic effect of CIK increased.With the prolong of DDP precondition time,the cytotoxic effect of CIK increased.Conclusion Preconditioning non-Hodgkin lymphoma cells with DDP can enhance the killing effect of CIK.