响应面分析法优化潲水油中胆固醇的提取条件

Optimization of Extraction of Cholesterol from Hogwash Oils Using Response Surface Methodology

利用响应面分析法对潲水油中胆固醇提取条件进行了优化,探讨了不同的提取剂、皂化温度、皂化时间以及KOH-乙醇浓度对油脂中包含的胆固醇提取效果的影响,以期最大限度地将油脂中的胆固醇提取出来,最终确定其最优条件为:提取剂为正己烷,皂化温度67℃、皂化时间60 min、KOH-乙醇浓度0.9 mol/L。

In order to get the maximal extraction content of cholesterol in oil, the effect of different extraction solvents, saponifica- tion temperature, saponification time and consistency of KOH-C2HSOH were discussed to optimize the cholesterol extraction conditions with the response surface methodology. Then the authors got the optimal conditions as follows： the extraction solvent was N-hexane, the extraction temperature was 67℃, the extraction time was 60min, the consistency of KOH-C2HSOH was 0.9 mol/L.