期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

河南商品蛋鸡群马立克氏病病毒流行株的分离与鉴定 被引量：2

Isolation and Identification of Field Strains of Marek's Disease Virus from Henan Province
下载PDF
导出
摘要 2011年～2012年，河南省多个地区养殖场鸡群爆发了马立克氏病，应用外周血淋巴细胞培养分离技术对流行毒株分离并进行了PCR鉴定，最终分离到17个马立克氏病毒毒株，这些病毒株感染鸡胚成纤维细胞3～5d后均能形成典型的病毒蚀斑，从感染细胞总DNA中均能扩增出132bp重复序列的特异性条带，且各毒株间132bp重复序列扩增产物的拷贝数存在明显差异，分离鉴定表明：河南鸡群中可能存在不同毒力马立克氏病毒野毒株的共流行。 Marek's disease( MD) was outbroken in vaccinated chickens in Henan province of China during 2011 ~ 2012. Using chicken embryo fibroblasts( CEF),17 filed isolates were obtained from diseased chickens. The isolates adapted to CEFs very well and most produced typical plaque at 3 ~ 5 d post-infection. Polymerase chain reaction( PCR) amplification of the 132 bp repeats sequence from the viral genome verified that all the isolates are Marek's disease virus( MDV). However,the copies of 132 bp repeats are apparently different in distinct isolates. The filed MDVs of differing virulence may widely spread in the central regions of Henan prorince.
作者 余祖华 丁轲 滕蔓 宿靖伟 罗俊
机构地区 河南科技大学动物疫病与公共安全重点实验室 河南省农业科学院农业部动物免疫学重点实验室 河南省农业科学院河南省动物免疫学重点实验室
出处 《河南科技大学学报（自然科学版）》 CAS 北大核心 2014年第1期67-70,6,共4页 Journal of Henan University of Science And Technology:Natural Science
基金 国家自然科学基金面上项目(31072145 31372445)
关键词 马立克氏病病毒 分离 鉴定 132 bp重复序列 Marek's disease virus isolation identification 132 bp repeat sequence
分类号 S852.65 [农业科学—基础兽医学] Q78 [生物学—分子生物学]
  • 相关文献

参考文献18

  • 1殷震;刘景华.动物病毒学[M]{H}北京:科学出版社,19971051-1066.
  • 2Biggs P M,Churchill A E,Rootes D G. The Etiology of Marek' s Disease-an Oncogenic Herpestype Virus[J].Perspect Virol,1968.211-237.
  • 3Buckmaster A E,Scott S D,Sanderson M J. Gene Sequence and Mapping Data from Marek' s Disease Virus and Herpesvirus of Turkeys:Implications for Herpesvirus Classification[J].{H}Journal of General Virology,1988.2033-2042.
  • 4Witter R L. Increased Virulence of Marek' s Disease Virus Field Isolates[J].{H}Avian Diseases,1997,(01):149-163.
  • 5Witter R L,Calnek B W,Buscaglia C. Classification of Marek' s Disease Viruses According to Pathotype:Philosophy and Methodology[J].{H}Avian Pathology,2005.75-90.
  • 6Lee L F,Wu P,Sui D. The Complete Unique Long Sequence and the Overall Genomic Organization of the GA Strain of Marek ' s Disease Virus[J].{H}Proceedings of the National Academy of Sciences(USA),2000,(11):6091-6096.
  • 7Tulman E R,Afonso C L,Lu Z. The Genome of a Very Virulent Marek' s Disease Virus[J].{H}Journal of Virology,2000,(17):7980-7988.
  • 8Cebrian J,Kaschka-Dierich C,Berthelot N. Inverted Repeat Nucleotide Sequences in the Genomes of Marek's Disease Virus and the Herpesvirus of the Turkey[J].{H}Proceedings of the National Academy of Sciences(USA),1982.555-558.
  • 9Osterrieder N,Kamil J P,Schumacher D. Marek' s Disease Virus:from Miasma to Model[J].{H}Microbiology,2006,(04):283-294.
  • 10Venugopal K. Marek' s Disease:an Update on Oncogenic Mechanisms and Control[J].{H}Research in Veterinary Science,2000.17-23.

二级参考文献48

  • 1张纪元,丁家波,崔治中,姜世金,Bublot Michel.不同致病型马立克氏病病毒1.8kb基因家族序列比较[J].中国病毒学,2005,20(3):277-282. 被引量：6
  • 2付本懂,王鲁,韦旭斌.马立克病病毒致瘤相关基因的研究进展[J].中国兽医科学,2006,36(7):592-596. 被引量：13
  • 3张颖,刘长军,秦运安,张艳萍,张晓巍,郝永清.应用双重实时荧光定量PCR方法检测鸡马立克氏病血清1型病毒[J].中国预防兽医学报,2007,29(1):46-51. 被引量：16
  • 4Brown A C, Baigent S J, Smith L P, et al. Interaction o1 MEQ protein and C-terminal -binding protein is critical for induction of lymphomas by Marek's disease virus[J]. PNAS, 2006, 103(6): 1687-1692.
  • 5Levy A M, Gilad O, Xia L, et al. Marek's disease virus Meq transforms chicken ceils via the V-Jun transcriptional cascade: A converging transforming pathway for avian oncoviruses[J]. PNAS, 2005, 102(41): 14831-14836.
  • 6Maotani K, Kanamori A, Ikuta K, et al. Amplification of a tandem direct repeat within inverted repeats of the Marekts disease virus DNA during serial in vitro passage [J]. J Virol, 1986, 58: 657-660.
  • 7Jarosinski K W, OrConnell P H, Schat K A. Impact of deletions within the Bam HI-L fragment of attenuated Marek's Disease Virus on vIL-8 Expression and the newly identified Transcript of Open Reading Frame LORF4 [J].Virus Genes, 2003, 26: 255-269.
  • 8Jarosinski K W, Osterrieder N, Nair V K, et al. Attenuation of Marek's Disease Virus by Deletion of Open Reading Frame RLORF4 but Not RLORF5a[J]. J Virol, 2005, 79: 11647-11659.
  • 9Camp H S, Coussens P M, Silva R F, et al. Cloning, Sequencing, and Functional Analysis of a Marek's Disease Virus Origin of DNA Replication[J]. J Virol, 1991, 65: 6320-6324.
  • 10Bradley G, Hayashi M, Lancz G, et al. Structure of the Marek's disease virus BamHI-H gene family: genes of putative importance for tumor induction[J]. J Virol, 1989, 63: 2534-2542.

共引文献32

同被引文献17

  • 1Bradley G, Hayashi M,Lanez G,et al. Structure of the Marek~s disease virus BamH I-H gene family~ Genes of putative importance for tumor induction[J]. J Vir- ol, 1989,63 : 2534-2542.
  • 2Gimeno I M,Witter R L,Hunt H D,et al. The pp38 gene of Marek' s disease virus (MDV) is necessary for cytolytic infection of B cells and maintenance of the transformed state but not for cytolytic infection of the feather follicle epithelium and horizontal spread of MDV[J]. J Virol, 2005,79 ( 7):4545-4549.
  • 3Kanamori A, Nakajima K, Ikuta K, et al. Copy number of tandem direct repeats within the inverted repeats of Marek~ s disease virus DNA[J]. Biken J, 1986, 29: 83-89.
  • 4Silva R F. Differentiation of pathogenic and non-path ogenic serotype Marek ~ s disease viruses (MDVs) by the polymerase chain reaction amplication of the tan- dem direct repeats with in the MDV genome[J]. Avi- an Dis, 1992,36 : 521-528.
  • 5Becker Y, Asher Y, Tabor E, et al. Polymerase chain reaction for differentiation between pathogenic and non-pathogenic serotype 1 Marek~ s disease viruses (MDV) and vaccine viruses of MDV serotypes 2 and 3[J]. J Virol Meth, 1992,40 : 307-322.
  • 6Levy A M, Izumiya Y, Brunovskis P, et al. Character- ization of the chromosomal binding sites and dimeriza- tion partners of the viral oncoprotein meq in Marek's disease virus transformed T cells[J].J Virol, 2003, 77(23) :12841-12851.
  • 7张艳萍,刘长军,施维松,秦运安,张晓巍,李晶梅.一株鸡马立克氏病毒的分离鉴定及其主要致病基因分析[J].中国预防兽医学报,2008,30(2):109-112. 被引量：21
  • 8施维松,刘长军,张艳萍,秦运安,张晓巍,李晶梅,陈洪岩.4株鸡马立克氏病病毒国内分离株Meq基因的克隆与序列分析[J].病毒学报,2008,24(2):117-125. 被引量：18
  • 9韦平.马立克氏病研究的最新动态——第八届国际马立克氏病研讨会概况[J].中国家禽,2008,30(17):6-14. 被引量：13
  • 10陈欣虹,秦爱建,钱锟,卢占军,张晨飞,苏钰文,邵红霞,金文杰.两株马立克病病毒的分离及相关致病基因序列的比较[J].中国兽医科学,2009,39(7):570-574. 被引量：5

引证文献2

二级引证文献10

河南科技大学学报（自然科学版）
2014年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部