不同类型精浆抗精子抗体对精液质量的影响

Impact of different types of antisperm antibodies in seminal plasma on the quality of semen

目的探讨不同类型精浆抗精子抗体对精液质量的影响。方法采用酶联免疫吸附法(ELISA)检测180例不育患者血清抗精子抗体,根据检测结果,选出精浆抗精子抗体单项阳性患者,分为B组(IgG阳性),C组(IgA阳性)和D组(IgM阳性),同时选用同期接受体检具有正常生育能力的21例男性作为对照组(A组)。比较4组精液质量的相关指标。结果①精浆抗精子抗体阳性(包括单项和多项)患者共107例,占不育症患者的59.4%;单项阳性患者62例,占抗体阳性患者的57.9%。②A组的精子密度、精子活动率、精子活力(a+b)、精子畸形率和精子液化时间等指标均显著优于B、C、D组(P<0.01)。C组的精子密度、精子活动率、精子活力(a+b)和精子液化时间均显著优于B、D组(P<0.01),精子畸形率显著优于B组(P<0.01),优于D组,但差异无统计学意义(P>0.05)。B组的精子活动率和精子活力(a+b)显著好于D组(P<0.01),精子畸形率和精子液化时间显著差于D组(P<0.01),精子密度差于D组,但差异无统计学意义(P>0.05)。结论抗精子抗体对于精液质量具有重要的影响,IgG对精子畸形率和液化时间的影响较大,IgM对精子活力(a+b)和精子活动率的影响较大。

Objective To investigate the impact of different types of antisperm antibodies in seminal plasma on quality of semen. Methods Antisperm antibodies in seminal plasma in 180 patients with infertility were detected by ELISA, and patients with single positive antisPerm antibodies in seminal plasma were selected according the detection results and these patients were divided into the group B （positive IgGpositive IgG positive IgG positive IgG）,the group C （positive IgA） the group D （positive IgM）,and 21 cases of male was chosen as the control group （the group A） who attended a med- ical examination had a normal fertility.The related indicators of quality of semen among the four groups. Results （~）An- tisperm antibodies in seminal plasma in I07 cases （including single and multiple） were positive,and it accounted for 59.4% in patients with infertility;antisperm antibodies in seminal plasma in 62 cases （single positive）,and it accounted for 57.9.% in patients with positive antibodies.（~）The sperm density,sperm activity rate,sperm motility （a＋b）, rate of ter- atosperm and sperm liquefaction time and other indicators in the group A were obviously better than that in the group B,the group C and the group D （P〈0.01）.The sperm density,sperm activity rate,sperm motility （a＋b） and sperm liquefac- tion time in the group C were obviously better than that in the group B and the group D （P〈0.01）,and the rate of ter- atosperm in the group C was obviously better than that in the group B （P〈0.01）,while the rate of teratosperm in the group C was obviously better than that in the group D,but there was no statistical difference between the two groups（P〉 0.05）.The sperm activity rate and sperm motility （a＋b） in the group B were obviously better than that in the group D （P〈0.01）,the rate of teratosperm and sperm liquefaction time in the the group B were obviously worse than that in the group D （P〈0.01）,the sperm density in the group B were worse than that in the group D,but there was no statistical dif- ference between the two groups （P〉0.05）. Conclusion Antisperm antibodies have an important impact on quality of se- men,IgG has a relatively large impact on sperm deformity rate and liquefaction time,IgM have a relatively large impact on sperm motility （a＋b） and sperm activity rate.