摘要
为了解厚颌鲂(Megalobrama pellegrini)的细胞遗传学特征,分析了厚颌鲂的染色体核型并对其DNA含量进行了测定。采用PHA和秋水仙素活体注射、肾细胞短期培养和空气干燥制片法,经Giemsa染色,对厚颌鲂染色体数目和核型进行研究,结果表明:厚颌鲂2n=48,染色体核型公式为24m+20sm+4st,臂数NF=92,具有1对特大染色体,未发现性染色体。以厚颌鲂外周血细胞为样本,鸡血细胞DNA为标准(2.30pg),使用流式细胞仪测定了厚颌鲂二倍体细胞的DNA含量,厚颌鲂DNA含量为鸡血的1.23倍,绝对含量为2.82pg。
In order to investigate the cytogenetic characters of Megalobrama pellegrini, the karyo- type and DNA content were studied in the present study. The chromosome number and karyotype were analyzed by a routine method including intraperitoneal injection of PHA and colchicine, cultivation invi- vo,and slides preparation with air-drying and Giemsa staining. The results showed that M. pellegrini had a chromosome number of 48 （2n=48）,with a large and long chromosome pair and no heterosome, and the karyotype was 2n= 24m＋20sm＋4st, NF= 92. Erythrocyte nuclear DNA content of M. pellegri- ni was determined by flow cytometry using the chicken erythrocytes （DNA content is 2. 30 pg） as a standard. The DNA content of M. pellegrini is 2. 82 pg and the ratio of DNA content between M. pelle- grini and chicken is 1.23.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2014年第1期80-84,共5页
Journal of Huazhong Agricultural University
基金
现代农业产业技术体系建设专项(CARS-46-05)
中央高校基本科研业务费专项(2011PY023)
关键词
厚颌鲂
染色体
核型
DNA含量
Megalobrama pellegrini
chromosome
karyotype
DNA content
