黏液脓性宫颈炎的病原体检测研究

Study on pathogens detection in patients with mucopurulent cervicitis

目的探讨黏液脓性宫颈炎(MPC)的病原体检出情况,以利其防治。方法采用白带常规镜检(悬滴法、涂片革兰染色)及荧光定量PCR检测方法,对250例MPC患者和120例正常人群,采集阴道及宫颈分泌物分别检测细菌性阴道病(BV)、阴道毛滴虫(TV)、假丝酵母菌(CA)、沙眼衣原体(CT)、解脲脲原体(UU)、淋球菌(NG)。结果 85.60%(214/250)的MPC患者和30.83%(37/120)的对照组查出病原体感染,两者差异有显著统计学意义(P=0.000)。MPC的UU、CT、NG、TV、BV检出率均明显高于对照组,差异均有统计学意义(P<0.05)。两组的CA检出率差异无统计学意义(P=0.10)。MPC的混合感染率(23.60%)明显高于对照组(3.33%)(P=0.000)。MPC混合感染前三位依次是(构成比):CT+UU(32.20%);CT+NG(16.95%);CT+UU+NG(13.56%)。结论 UU、CT、NG、TV、BV在MPC的发病中占重要作用,CA感染可能与MPC无关。明确MPC的病原体检出情况,对其防治有指导作用。

Objective To discuss the pathogens detection condition in patients with MPC, so as to prevent and control MPC. Methods With the routine microscopic examination （hanging drop method and smear gram staining） and FQ - PCR, vaginal and cervical secretions were collected from 250 MPC patients and 120 healthy controls to detect BV, TV, CA, CT, UU and NG. Results The pathogen positive rate of MPC patients was 85.60% （214/250） and that of healthy controls was 30.83% （37/120）, and the difference had obvious statistical significance between the two groups （P = 0. 000）. The detection rates of UU, CT, NG, TV and BV in MPC patients were significantly higher than those in the control group （ P 〈 0.05）. However, there was no significant statistical difference of the CA detection rate between the two groups （P = 0.10）. The mixed infection rate of MPC （23.60%） was significantly higher than that of the control group （3.33 % ） （P = 0. 000）. In the mixed infection of MPC , the top three were CT＋UU（32.20%）, CT＋NG（16.95%）, CT＋UU＋NG（13.56%）. Conclusion UU, CT, NG, TV and BV play an important role in incidence of MPC. There may be no correlation between CA infection and MPC. Identification of the pathogens of MPC can guide the prevention and control of this disease.