南阳市耐多药结核(MDR-TB)rpoB基因突变分子特征研究

Molecular analysis of rpoB gene mutation in multidrug- resistant tuberculosis( MDR- TB) isolates from Nanyang

目的研究南阳市耐多药结核菌(MDR-TB)利福平耐药相关的rpoB基因突变分子特征。方法特异性扩增172株MDR-TB rpoB基因耐药决定区(81 bp)并进行基因测序。结果有157株(91.2%)在耐药决定区发生了突变,最常见的三种突变类型是531(TCG-TTG),526(CAC-GAC)和533(CTG-CCG),突变率分别为41.3%,11.6%和10.5%。结论 rpoB基因耐药决定区(81 bp)的基因突变是引起南阳市结核分枝杆菌利福平耐药的主要原因。

Objective To reveal the molecular characteristics of rpoB gene mutation associated with rifampicin resistance in muhidrug - resistant tuberculosis in Nanyang. Methods One hundred and seventy - two muhidrug - resistant tuberculosis （ MDR - TB） strains were involved to amplify the rpoB gene at rifampin resistance determining region（81 bp） by PCR, then the PCR products were sequenced. Results Mutations in the rpoB were identified in 91.2% of the isolates （157/172）. Nucleotide substitutions in codons 531, 526, 533 （41.3% , 11.6% and 10.5%, respectively） were the most prevalent mutations. Conclusion Refampin resistance was mainly caused by mutation of the 81 - bp region of rpoB gene in mycobacteria tuberculosis isolates from Nanyang.