转化生长因子β对人牙周膜成纤维细胞的生物学作用

The biological effects of transforming growth factor-β on human periodontal ligament fibroblasts

目的 研究转化生长因子 β(transforminggrowthfactor β ,TGF β)对人牙周膜成纤维细胞(humanperiodontalligamentfibroblasts,HPDLFs)的生物学作用。方法 原代培养HPDLFs,并观察TGF β对HPDLFs的增殖、碱性磷酸酶 (alkalinephosphatase ,ALP)活性、骨钙素 (osteocalcin ,OC)合成及矿化结节形成的作用。结果 0 0 0 5 0～ 0 10 0 0mg/LTGF β可明显刺激HPDLFs的增殖 ,0 0 0 5 0mg/LTGF β可显著提高HPDLFs的ALP活性 ,0 0 0 0 5～ 0 10 0 0mg/LTGF β对HPDLFs合成OC和矿化结节的形成无明显影响。结论 TGF β对HPDLFs的作用呈剂量依赖性。TGF β能够刺激HPDLFs表达某些成骨细胞表型 ,但不能促进成骨表型的成熟。

Objective To evaluate the biological effects of transforming growth factor β (TGF β) on human periodontal ligament fibroblasts (HPDLFs). Methods HPDLFs were primary cultured and examined for the effects of TGF β on its proliferation, alkaline phosphatase (ALP) activity, osteocalcin (OC) synthesis and formation of the mineralized nodules. Results TGF β(0 005 0～0 100 0 mg/L) significantly stimulated the proliferation of HPDLFs. The ALP activity of HPDLFs was elevated evidently by 0 005 0 mg/ L TGF β. TGF β (0 000 5～ 0 100 0 mg/L) had no effects on OC synthesis and formation of the mineralized nodules of HPDLFs. Conclusions The effects of TGF β on HPDLFs are dose dependent. TGF β can stimulate HPDLFs to express some of osteoblastic phenotype, and it lacks the ablility to promote maturation of the osteogenic phenotype.