人CD80-Linker-SEA重组毒素的设计及生物学特性预测

The design and prediction of biology characteristics of recombinant toxin CD80-Linker-SEA

目的 :构建人重组CD80 Linker SEA毒素的真核表达载体并预测Linker的合理性和可行性。方法 :应用核酸和蛋白质序列分析软件GolgKey对融合基因及Linker部位翻译后在二级结构水平上的一些生物学特性 ,诸如柔性、抗原性、亲水性及表位等加以预测。结果 :CD80 Linker SEA融合基因的氨基酸序列经软件分析 ,并分别与CD80和SEA单独的分析结果相比较 ,发现在二级结构的水平未出现新的抗原性 ,同时在Linker部位具有很低的抗原性 ,亲水性没有改变 ,Linker部位呈中性 ,CD80和SEA具有原来各自的表位特征 ,无新的表位出现。结论 :本研究通过计算机软件对CD80 Linker SEA融合蛋白的预测 ,并与CD80、SEA各加以对比分析和比较 ,以利于在重组过程中有一个合理的设计 。

Objective:To construct the eukaryotic expressed vector which express recombinant toxin CD80 Linker SEA and predict the rationality and feasibility of the linker Methods:Utilize the sequence analysis software to analyze the flexibility、antigenicity、Hoop&Woods hydrophilicity and episode of recombinant toxin CD80 Linker SEA Results:Through the analysis of the software,it could be found that the recombinant toxin has correct domains of CD80 and SEA The linker has low episode、low antigenicity and high flexibility Conclusion:The results of computer analysis could help us to rationally design the recombinant toxin CD80 Linker SEA and keep it's maximum biological activity