胃癌组织中尿激酶型纤溶酶原激活剂及其受体基因表达和临床意义

Expression and clinical significance of urokinase-type plasminogen activator and its receptor in gastric cancer

目的 :研究尿激酶型纤溶酶原激活剂 (uPA)及其特异受体 (uPAR)与胃癌的关系及其在胃癌浸润转移中的作用。方法 :采用cDNA mRNA原位分子杂交技术 ,分别检测了 6 4例胃癌及其癌旁组织中uPA和uPARmRNA表达情况 ,同时结合病人的临床生物学指征进行分析。结果 :癌与癌周比较 ,uPA和uPARmRNA阳性表达率明显升高 ,P <0 0 0 1。在伴有淋巴结转移的病例中 ,uPA和uPAR阳性例数分别为 19 2 9和 2 4 2 9,与无转移的 10 35和 13 35相比 ,P 分别 <0 0 1和 <0 0 0 1。在浸润到肌层、浆膜层的病例中uPA和uPAR阳性例数分别为 2 5 38和 30 38,与浸润到粘膜和粘膜下层的 4 2 6例和 7 2 6相比 ,P均 <0 0 0 1。在uPA和uPAR同时阳性的病例中 ,伴有淋巴结转移和浸润到肌层以下的分别占 15 2 0和 16 2 0 ,与阴性组 3 11和 1 11相比 ,P <0 0 1。结论 :uPA、uPARmRNA表达在胃癌中明显升高 。

Objective:To investigate the expression of urokinase type plasminogen activator (uPA) and its receptor(uPAR) mRNA in gastric cancer tissues and the relationship with tumor cell invasion and metastasis Methods:Paraffin embedded biopsies from 64 patients with gastric cancer were detected for uPA and uPAR mRNA by in situ hybridization technique Results:When compared to gastric cancer adjacent tissue,positive rates of staining for uPA and uPAR were significantly higher in gastric cancer cells(P<0 001) Positive staining of uPA and uPAR was seen in 19 of 29 and 24 of 29 cases of gastric cancer with lymph node metastasis,respectively(P<0 01 and <0 001) Positive staining of uPA and uPAR was seen in 25 of 38 and 30 of 38 cases of gastric cancer invaded into muscularis and serosa(P<0 001) In 20 cases positive for all two parameters , 15 had cancer with lymph node metastasis and 16 had invaded under muscularis(P<0 01) Conclusion:Expression of uPA and uPAR mRNA is increased in gastric cancer,and uPA and uPAR may contribute significantly to gastric cancer invasion and metastasis