尖顶羊肚菌菌丝体胞外多糖的分离纯化及结构研究

Isolation and characterization of an extra-cellular polysaccharide from Morchella conica fermentation broth

用乙醇沉淀、Sevag法除蛋白等方法得到羊肚菌菌丝体胞外粗多糖,并进一步通过离子交换色谱、分子筛色谱对粗多糖进行分离纯化,得到单一多糖组分MEP3A.经外光谱法、紫外光谱法、核磁共振法等仪器分析方法确定该多糖组分结构为直链→6)-α-D-Man p-(1→,分子量81.2kDa,不含蛋白质、多肽和氨基酸.采用MTT法检测表明,该多糖组分可促进小鼠脾淋巴细胞增殖,具有免疫调节活性.

The crude polysaccharides were extracted by alcohol-precipitation and deproteinized by Sevag methodfrom Morchellaconica fermentation broth .A fraction of polysaccharides （namely MEP3A） were then further purified by DEAE-cellulose ion exchange and gel permeation chromatography .The structur-al characteristicsof MEP3A was identified to an →6）-α-D-Man p-（1→ with the molecular weight of about 81 .2 kDa by HPLC-ELSD ,IR and NMR spectroscopy .It was found that MEP3Acould promote the spleen lymphocyte proliferation in a dose -dependent manner .