卵巢恶性上皮性肿瘤中树突状细胞的免疫组织化学研究

Immunohistochemical study on dendritic cells in epithelial ovarian cancer

目的 探讨树突细胞在卵巢恶性上皮性肿瘤中的浸润情况及其临床意义。方法 用S -10 0蛋白抗体对48例卵巢恶性上皮性肿瘤组织中间质树突细胞进行免疫组化S -P法标记染色 ,并与正常组织及卵巢良性上皮性肿瘤对照。结果 卵巢恶性上皮性肿瘤组织中树突细胞浸润明显增多 (P <0 .0 1) ,树突细胞浸润数量与组织类型 ,分化程度无关 ,而与淋巴细胞浸润呈直线相关 (P <0 .0 1)。树突细胞浸润程度与患者预后呈正相关 (P <0 .0 5 )。结论 树突细胞的浸润可能与肿瘤免疫有关 ,肿瘤组织原位树突细胞计数 。

Objective To study the infiltration of dendritic cells(DC) in epithelial ovarian cancer. Methods \ Quantitative assessment of interstitial dendritic cells was performed by S-P immunohistochemical method in specimens of maligmant epithelial ovarian tumor ( n =48),benign epithelial ovarian tumor( n =30),and normal ovarian tissue( n =30). Results\ The number of DC in maligmant epithelial ovarian tumor increased significantly as compared with benign epithelial tumor and normal ovarian tissue( P <0.01).The DC number in maligmant epithelial ovarian tumor was not correlated with histologic types and differentiation grades, but was correlated with lymphocyte infiltration and survival rate. Conclusion\ DC may play a role in the host immune reaction against tumor and the number of DC in situ can serve as a useful indicator in assessing prognosis.