腺病毒介导人B7-1基因对肝癌细胞免疫原性的影响

Effect of immunogenicity of liver cancer cell lines by transduction of adenovirus mediated human B7 1 gene

目的研究同时含人 p5 3、GM- CSF、B7- 1、IL- 2基因的重组腺病毒载体 Ad- m ultigenes,对肝癌细胞免疫原性的影响。方法应用人外周血淋巴细胞和肿瘤细胞的混合培养、外周血 T淋巴细胞杀伤活性试验 ,分析导入目的基因的人肝癌细胞免疫原性的变化。结果导入 Ad- m ultigenes的肝癌细胞系 Hep G2、BEL- 740 2 ,体外能刺激正常人外周血 T淋巴细胞的增殖 ,并能增强 T淋巴细胞的杀瘤细胞活性 ,加入 IL- 12有协同增强作用。结论肝癌细胞导入含 B7- 1基因的 Ad- m ultigenes后 。

Objective To investigate the change of immunogenicity of liver cancer cells transduced with adenovirus mediated human 4 kind of genes (p53,GM CSF,B7 1,IL 2).Methods Using human mixed lymphocyte of peripheral blood and tumor cell coculture,cytotoxicity assay of peripheral blood T lymphocyte,we studied the change of immunogenicity of liver cancer cells transduced with Ad multigenes.Results The proliferation of human peripheral blood T lymphocytes and T lymphocyte cytotoxicity activity against parent tumor cells were increased by transduction of Ad multigenes in liver cancer cells HepG2 and BEL 7402.IL 12 has a strong synergetic effect with Ad multigenes in inducing proliferation and cytotoxicity of T cells.Conclusion The immunogenicity of liver cancer cells was enhanced after transduction of adenovirus mediated multigenes which containing B7 1 molecule.