抗CD28+B7.1单抗共刺激PBLs诱导肝癌细胞凋亡的蛋白激酶信号转导

<etitle><Title>The signal transduction of protein kinase in PBLs co stimulated by anti CD28+B7 1 McAb and the induction of apoptosis of the hepatoma cells<eauthor><Author>YANG Hong 1, CHEN Geng fu 1, HUANG Yu shan 2, HUANG Shu lin 1 (1 Dep

目的研究 T淋巴细胞活化、增殖、介导肝癌细胞凋亡过程中其蛋白激酶 C(PKC)和酪氨酸蛋白激酶 (TPK)的活性变化。方法用抗 CD2 8+ B7.1(CD80 )单克隆抗体共刺激正常人外周血淋巴细胞 (PBL s)后作用于肝癌细胞 (BEL -740 2 )。结果激活的 T细胞中 PKC、TPK活性明显增强 ,并与肝癌细胞凋亡程度呈正相关。结论 PKC、TPK在 T细胞活化。

ObjectiveTo investigate the activities of protein kinase C (PKC) and tyrosine protein kinase (TPK) in the activated and proliferating T cells and the induction of apoptosis of the hepatoma cells MethodsHealthy human PBLs were co stimulated by anti CD28 and anti CD80 (B7 1) McAb and acted on the hepatoma cells (BEL 7402) ResultsThe activities of PKC and TPK were significantly higher in the activated T cells than those in the control cells, and were positively related to apoptosis of hepatoma cells ConclusionPKC and TPK play important role on the signal transduction in the activated and proliferating T cells and the induction of apoptosis of hepatoma cells<ekeyword><