App17肽对Tau蛋白超磷酸化表达的影响

THE EFFECT OF App17 PEPTIDE ON THE EXPRESSION OF HYPERPHOSPHORYLATED Tau PROTEIN

目的 通过对 Tau蛋白磷酸化在脑内分布的观察 ,研究 App17肽对糖尿病小鼠脑神经元 Tau蛋白磷酸化的影响。 方法 用链脲佐菌素 (streptozotion,STZ)诱发小鼠糖尿病模型 ,并皮下注射 App17肽对糖尿病小鼠进行治疗 ,4周后取脑组织做 AT- 8、Tau- 1、蛋白磷酸酯酶 (PP- 2 B)脱磷酸后的 Tau- 1免疫组织化学染色。 结果 糖尿病小鼠脑内 AT- 8阳性反应神经元广泛分布于压后颗粒皮层、海马、丘脑、下丘脑等部位 ,胞浆深染 ,而正常小鼠及 App17肽保护的糖尿病小鼠脑内阳性反应细胞仅在海马和压后颗粒皮层表达 ,阳性细胞数目少 ,染色淡 ;用Tau- 1单染正常小鼠脑及 App17肽治疗的糖尿病小鼠脑的压后颗粒皮层及海马阳性反应神经元数目多 ,糖尿病小鼠只有用 PP- 2 B脱磷酸后阳性反应神经元数目及染色程度才与正常组接近。 结论 糖尿病小鼠脑内 Tau蛋白磷酸化广泛表达 ,App17肽可改善糖尿病小鼠脑内 Tau蛋白磷酸化 ,从而改善学习与记忆。

Objective Through the observation on the distribution of hyperphosphorylated Tau,to investigate the connection between hyperphosphorylated Tau and learning, memory tasks. Furthermore, the treatment of App17 on brain tissues of diabetic mice. Methods Diabetic model mouse was produced in the use of streptozotion and App17 peptide as a curative was injected subcutaneously. Four weeks later, removed the brains. Immunohistochemical stainning was done with AT\|8, Tau\|1, again with Tau\|1 antibody after dephosphorylation. Results In the brains of diabetic mice positive AT\|8 reacting neurons were widely distribution in retrosplenial granular cortex, hippocampas, thalamus et al, the cytoplasm was darkly stained, while in normal mice and App17 peptide\|treated diabetic mice positive cells were localized in retrosplenial granular cortex, however, in hippocampas and RSG area, the cytoplasm were poorly stained. Conclusion Hyperphosphorylated Tau is widely expressed in brains of diabetic mice. App17 peptide can improve the hyperphosphorylated Tau in brains of diabetic mice, therefore, it may improve learning ability and memory.\;