一氧化氮合酶在自发性高血压大鼠脊髓中间带灰质的分布

THE DISTRIBUTION OF NOS POSITIVE NEURONS AND FIBERS IN SPINAL INTERMEDIATE GRAY OF SPONTANEOUSLY HYPERTENSIVE RATS

目的 探讨自发性高血压大鼠脊髓中间带灰质内一氧化氮合酶阳性细胞和纤维的分布及其与自发性高血压的关系。 方法 采用 NADPH- d组织化学方法 ,并以计算机图像分析系统对脊髓胸腰部中间外侧核本部区 NADPH- d染色强度 (A值 )进行半定量分析。 结果 1.在脊髓中间带灰质区 ,一氧化氮合酶阳性神经细胞和纤维主要见于胸腰部中间外侧核本部区和中央管周围 ,在中介核区也有少量分布。胸腰部中间外侧核本部区染色阳性细胞成群分布 ,细胞群的大小、间距随节段而稍有不同。一氧化氮合酶阳性细胞分布模式在自发性高血压大鼠和正常鼠两组之间未发现明显差异。 2 .在脊髓 T2 、T5 、T8、T1 1 、L1 各节段中间外侧核本部区 ,一氧化氮合酶阳性物质的 A值 ,自发性高血压大鼠比正常鼠低。统计结果显示 ,差异有显著性 (P<0 .0 5 )。 结论 脊髓中间带灰质的交感神经节前神经细胞可能通过一氧化氮表达参与调控血压升高和维持。

Objective To investigate the distribution of nitric oxide synthase (NOS) in the spinal intermediate gray and it's relationship with hypertension. Methods Using NADPH\|d histochemistry method, the distribution of NOS in the spinal intermediate gray was investigated under microscope and analyzed quantitatively with image analysis system. Results 1 NOS positive neurons were observed mainly in the nucleus intermediolateralis thoracolumbalis pars principalis(ILp) and paracentral gray, as well as in the area of nucleus intercalatus spinalis. NOS positive cells existed in groups in ILp. The size and density of NOS positive cell groups differed with different spinal segments, no significant difference could be found in the distribution pattern of NOS positive neurons in the spinal intermediate gray between SHR and WKY. 2 The A value of NOS positive substance in the ILp of SHR was significantly lower than that in WKY. Conclusion The results imply that lower expression of NO in the area of ILp in SHR might be related to the development and maintenance of hypertension in SHR.\;