bcl-2基因在小鼠睾丸及试验隐睾中表达的研究

THE STUDY OF bcl-2 GENE EXPRESSION IN NORMAL MOUSE TESTES AND EXPERIMENTAL CRYPTORCHIDISM

目的 研究 bcl- 2基因在正常小鼠睾丸中的定位表达及试验隐睾所导致的改变。 方法 以 West-ern- blotting印迹法从蛋白水平检测 bcl- 2基因在正常小鼠睾丸及试验隐睾中的表达、变化 ;地高辛标记 bcl- 2基因c DNA探针 ,石蜡组织切片原位杂交技术 ,从 m RNA水平检测 bcl- 2基因在小鼠生精上皮中的定位及试验隐睾所导致的改变。 结果 Bcl- 2蛋白在正常小鼠睾丸中有较丰富的表达 ,试验隐睾导致其表达明显降低 ;bcl- 2基因在生精细胞中有较高水平的 m RNA转录 ,表达细胞主要为各级生精细胞 ,支持细胞和间质细胞未见表达 ,隐睾术后仍然保持较高水平的 m RNA转录。 结论 Bcl- 2蛋白可能在生精细胞主动退化的调节中起着重要作用。

Objective\ To investigate that bcl 2 gene expresses and distributes in mouse testes and experimental cryptorchidism. Methods\ Western blotting and in situ hybridization with dig labeling bcl 2 cDNA probe were used. Results\ Bcl 2 protein expressed in normal mouse testes.In experimental induced cryptorchidism Bcl 2 protein decreased obviously.bcl 2 mRNA transcription kept high level in normal mouse testes and experimental cryptorchidism.bcl 2 gene expressed mainly in spermatocytes, spermatogonia and spermatids.Negative signals were seen in Sertoli cells and Leydig cells. Conclusion\ Bcl 2 protein may play an important role in germ cells degeneration.\;