大鼠肾上腺的NADPH、NPY、CGRP、SP、c-fos细胞化学特性

DISTRIBUTION OF NADPH,NPY,CGRP,SP,c-fos IN RAT ADRENAL GLAND:CYTOCHEMICAL STUDY

目的 探讨肾上腺内分泌组织和神经组织的双重组织学特性。 方法 用组织化学和免疫组织化学技术 ,在光学显微镜下观察 NADPH、NPY、CGRP、SP、c- fos在大鼠肾上腺的分布。 结果 肾上腺皮质分布有NPY阳性神经细胞和神经纤维、CGRP阳性神经纤维 ;肾上腺髓质分布有 NADPH - d阳性神经细胞和神经纤维、CGRP阳性神经纤维、SP阳性神经纤维、c- fos阳性神经细胞和神经纤维。肾上腺皮质球状带、网状带、束状带细胞均 NADPH- d阳性 ,髓质部分嗜铬细胞 NADPH - d阳性 ,部分嗜铬细胞 NPY阳性 ,部分嗜铬细胞 CGRP阳性 ,部分嗜铬细胞 SP阳性。 结论 大鼠肾上腺接受广泛的非经典递质的神经支配 ,特别是肽能神经支配的肾上腺实质细胞及髓质嗜铬细胞 ,能分泌多种神经肽物质。提示肾上腺的内分泌活动不仅受到复杂的神经调节而且也受到自身的活性物质的调节。

Objective\ The adrenal gland is a linkage between nervous system and endocrine system.Its cortex results from mesoderm,and medulla derives from neuroectoderm.Searching its double characteristic of nervous and endocrine tissue is our aim. Methods\ Histochemical and immunohistochemical staining techniques were used,and immunostained sections were observed under a light microscopy. Results\ NPY positive neuron and nerve fiber,CGRP positive nerve fiber existed in adrenal cortex.NADPH d positive neuron and nerve fiber, c fos positive neuron and nerve fiber,CGRP positive nerve fiber,SP positive nerve fiber were detected in adrenal medulla.NADPH d positive cell,NPY positive cell,CGRP positive cell,SP positive cell were found among the adrenal chromaffin cells.The zona glomerulosa,zone fasciculata and zona reticularis were stained strongly by NADPH d in cortex. Conclusion\ The adrenal gland was innervated by NO ergic,NPY ergic,CGRP ergic,SP ergic, c fos ergic nerves.The adrenal parenchyma,especially chromaffin cells can produce NO,NPY,CGRP,SP which regulate adrenal endocrine by itself.The nervous control of adrenal function and neuroendocrine function of adrenal gland is more complex than hitherto believed.\;