摘要
目的 探讨转移生长因子 (TGF) β、碱性成纤维细胞生长因子 (bFGF)对人成骨细胞血小板衍生生长因子 (PDGF) BmRNA表达的影响及其意义。方法 体外分离培养人成骨细胞。在体外培养的成骨细胞中分别加入 10、2 0、40、80、16 0 μg/L梯度浓度的PDGF BB培养细胞 2 4h ,以摄取3H TdR为细胞增殖指标检测细胞增殖状况。以 4μg /L的TGF β和 10 μg/L的bFGF培养细胞 2 4h ,寡核苷酸探针检测细胞PDGF BmRNA的表达。结果 10~ 16 0 μg/L的PDGF BB可促进成骨细胞增殖 (P <0 .0 5 )。在普通培养条件下 ,细胞不表达PDGF BmRNA ;当培养体系中加入TGF β和bFGF ,可见PDGF BmRNA的表达。结论 PDGF B基因的表达可能是骨组织生长的储备因素 ,TGF β和bFGF可诱导成骨细胞表达PDGF
Objective To investigate the effects of transforming growth factor β (TGF β) and basic fibroblast growth factor (bFGF) on the induction of platelet derived growth factor B (PDGF B) gene expression in human osteoblasts and implication. Methods The osteoblasts were isolated from human fetal calvaria. After the cells incubated with PDGF BB at serious concentrations of 0, 10, 20, 40, 80, 160 μg/L for 24 h, the incorporation of 3H TdR into these cells was investigated. The osteoblasts incubated with TGF β (4 μg/L) or bFGF (10 μg/L) for 24 h, and then PDGF B mRNA expression was detected by oligonucleotide probe. Results PDGF B(10~60 μg/L)could increase the incorporation of 3H TdR into human osteoblasts significantly ( P <0.05). PDGF B mRNA was not detectable in the osteoblasts in control group that incubated without FBS, but PDGF B mRNA was detectable in the cells incubated with TGF β or bFGF. Conclusion The expression of PDGF B gene acts as a spare factor to the growth of bone tissue. TGF β and bFGF have the capacity of inducing the expression of PDGF B gene. [
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2001年第1期72-73,共2页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目 !(3950 0 1 75)