大鼠脊髓损伤后一氧化氮合酶基因表达的变化

Changes of nitric oxide synthase gene expression in injured spinal cord tissue

目的 探讨大鼠脊髓损伤后 3种类型一氧化氮合酶 (NOS)mRNA表达的变化规律。方法 成年SD大鼠 36只 ,随机分为种类 6组 ,每组 6只大鼠。建立大鼠脊髓压迫伤模型 ,以逆转录 聚合酶链反应 (RT PCR)法测定伤段脊髓组织神经型 (nNOS)、诱导型 (iNOS)及内皮型 (eNOS)一氧化氮合酶的mRNA表达情况。结果 脊髓压迫伤后nNOSmRNA及NOSRNA表达增强 ,伤后 6h达到高峰 0 .6 33± 0 .0 12、1.2 36± 0 .2 0 7;iNOSmRNA表达亦增高 ,但在伤后 2 4h才达到高峰 1.0 43± 0 .0 49。结论 脊髓损伤后NOSmRNA的表达增强 ,但不同类型的NOSmRNA变化规律不同 。

Objective To study the changes of nitric oxide synthase (NOS) gene expression of its three isoforms in injured spinal cord tissue. Methods Thirty six adult Sprague Dawley rats were divided randomly into six groups: a normal group and five injured groups, six animals in each group. A compression injured model of rat spinal cord was established and the gene expression levels of neuronal NOS (nNOS),inducible NOS (iNOS) and endothelial NOS (eNOS) were measured by reverse transcription polymerase chain reaction (RT PCR). Results The expression of NOS mRNA was upregulated after injury: the expression of nNOS mRNA and eNOS mRNA was increased shortly after the injury and reached their peaks at 6 h [( 0.633 ± 0.012 ),( 1.236 ± 0.207 )] , while the expression of iNOS mRNA increased later and reached its peak at 24 h( 1.043 ± 0.049 ). Conclusion The NOS gene expression was increased after spinal cord injury in different ways, but the changes of different types of NOS mRNA were varied. Promation or inhibition of different NOS mRNA may alleviate the secondary spinal cord injruy. [