HPLC示差折光检测测定黄芪精口服液中黄芪甲苷的含量

Determination of astragaloside IV in the Huangqijing by HPLC RI

目的 建立用HPLC示差折光检测测定黄芪精口服液中黄芪甲苷含量的方法。方法 以HypersilODS 2 (4 .6mm× 2 0 0mm ,5 μm)色谱柱 ,甲醇 水 (6 7∶33)为流动相 ,流速 1mL·min-1。结果 线性范围 1～ 5 μg,r=0 .9999,加样回收率为 98.1% ,RSD =2 .0 2 % (n =6 )。结论 本法简单方便 ,重复性好 。

OBJECTIVE To establish a HPLC RI assay for the determination of astragaloside IV in the Huangqijing solution.METHODS The column Hypersil ODS 2(4.6 mm×200 mm,5 μm) was used.The mobile phase was consisted of methanol ane water (67∶33).The flow rate was 1 mL·min -1 .RESULTS The standard curve was linear over the range of 1～5 μg with the correlation coefficient 0.9999.The average recovery was 98.1% with the RSD 2.02%( n =6).CONCLUSION This method was sensitive,accurate,repeatable and easy to operate.