“藏茵陈”原植物及其混淆种类的ITS序列比较

A COMPARISON OF THE ITS SEQUENCES OF THE TIBETAN MEDICINE “ZANG YIN CHEN” — SWERTIA MUSSOTTI AND ITS ADULTERANT SPECIES

目的 比较藏药“藏茵陈”原植物川西獐牙菜及近年市场出现混淆种类的ITS序列 ,为分子序列鉴定提供参照系统并为寻找新的药用植物来源提供分子证据。方法 利用PCR扩增和ABI377自动测序。结果 川西獐牙菜不同居群间的序列相同 ,与其混淆种类之间存在一定的分子序列差异 ,有其特定的分子序列 ;利用Paup程序聚类分析表明川西獐牙菜与抱茎獐牙菜聚在一起。结论 ITS序列可正确鉴定川西獐牙菜与其混淆种类 ,他们之间的分子序列差异为进一步设计特异性分子鉴定试剂盒提供了科学依据 ;抱茎獐牙菜可考虑作为“藏茵陈”药材的新植物来源。

AIM To identify the medicine and search for new medicine resource at the molecular level, the ITS of “Zang Yin Chen” — Swertia mussotti and its adulterant species were sequenced. METHODS The double stranded DNA was amplified using PCR systems 9 600 kits and sequenced on an ABI 377 automated sequencer from both directions. RESULTS The ITS sequences of S.mussotti in different populations showed no variation. It has the unique ITS sequence and shows distinct difference from its adulterant species. In the phylogenetic tree based on the ITS data of S.mussotti and all vicarious species constructed by Paup, S.franchetiana and S.mussotti clustered together with high bootstrap support. CONCLUSION ITS sequences can be used for the molecular authentication between the S.mussotti and its adulterant species. S.franchetiana can be regarded as a new medicine resource of “Zang Yin Chen”.