失血性休克大鼠心肌收缩功能障碍中整合素联接激酶的作用

Role of ILK in the dysfunction of myocardium contraction following hemorrhagic shock

目的探讨整合素联接激酶(ILK)在失血性休克大鼠心肌收缩功能中的调节作用。方法采用离体乳头肌灌流和心脏灌流技术,用不同方法预处理ILK,测定失血性休克大鼠离体左心室乳头肌等长收缩张力的变化,以及大鼠离体心脏血流动力学指标的变化。结果休克大鼠心脏血流动力学指标随着休克时间延长而逐渐下降,休克1、2h的血流动力学指标显著低于对照组(P<0.05);离体左心室乳头肌收缩力呈现相同的变化趋势(P<0.05);休克0.5、1.0、2.0hILK活性显著低于对照组(P<0.05)。以休克1.0h的离体乳头肌和心脏为研究对象,ILK激动剂预处理可显著改善乳头肌的收缩力和心脏血流动力学指标(P<0.05),而ILK特异性抑制剂则明显拮抗这种改善作用(P<0.05)。结论 ILK在休克心肌收缩功能调节中发挥了重要作用,增强ILK的活性可增强心肌收缩功能。

Objective To explore the effect of ILK on myocardium contraction following hemorrhagic shock .Methods With iso-lated cardiac papillary muscle and isolated heart ,the contraction of papillary muscle and hemodynamic parameters （left intraventricu-lar systolic pressure（LVSP） ,the maximal change rate of left intraventricular pressure were measured .Results Compared with nor-mal control hearts ,ILK activity ,contractile response of cardiac papillary muscle and hemodynamic parameter were decreased signifi-cantly gradually in shock heart at the 1 ,2 h（P〈0 .05） ,and the change of ILK activity was positive correlative with contractile re-sponse and hemodynamic parametert .With ILK agonist [phosphatidylinositol（3 ,4 ,5）trisphosphate ,PLTP] the dysfunction of con-tractile response and hemodynamic parameter could be improved significantly （P〈0 .05） ,while these improvement could be abol-ished by ILK specific inhibitor（P〈0 .05） .Conclusion ILK play important role in the regulation of cardiac contractility following hemorrhagic shock .