阿托伐他汀对oxLDL诱导泡沫细胞Lkn-1表达的影响

The effect of atorvastatin on the expression of Lkn-1 during U937 monocyte differentiating into foam cells induced by oxidized LDL

目的观察阿托伐他汀对ox-LDL诱导U937细胞形成泡沫细胞过程中Lkn-1表达的影响,探讨阿托伐他汀抗AS的作用机制。方法在由0.1μmol/L佛波脂(PMA)诱导分化人U937巨噬细胞中加入100 mg/Lox-LDL及不同浓度(0.1、1、10μmol/L)的阿托伐他汀共同孵育24 h,分别用酶联免疫吸附试验(ELISA)和RT-PCR方法检测培养细胞上清中Lkn-1的表达变化。结果 ox-LDL组较正常对照组Lkn-1的表达明显增加(P<0.05)。给药各组较ox-LDL组Lkn-1明显减少(P<0.05)。且随阿托伐他汀浓度的增加Lkn-1表达呈逐渐减少的趋势(P<0.05)。结论阿托伐他汀能抑制ox-LDL诱导U937细胞系形成泡沫细胞过程炎症因子Lkn-1的表达和分泌,可能为其抗动脉粥样硬化的重要机制之一。

Objective To observe the effect of atorvastatin （AT） on the expression of Lkn-1 during U937 monocyte differentiating into foam cells induced by oxidized LDL （ox-LDL） ,and to explore the antiatherosclerotic action mechanism of AT. Methods The macrophages derived from U937 monocytes were induced by PMA （0.1μmoL/L） , which were treated with 100mg/L ox-LDL plus different concentrations of AT （0.1,1,10μmol/L） for 24 hours. Then the expression levels of Lkn-1 in supernatant of cultured cell were detected by ELISA and RT-PCR. Results As compared with those in control group, the expression levels of Lkn-1 of in ox-LDL group were significantly increased （ P 〈 0.05 ）. However the expression levels of Lkn-1 in different concentrations of AT groups were significantly decreased, as compared with those in ox-LDL group （ P 〈 0.05 ）. Moreover with the increase of AT concentration, the the expression levels of Lkn-1 were gradually decreased （ P 〈 0. 05 ）. Conclusion Atorvastatin can inhibit the expression and secretion of Lkn-1 in the course of U937 monocyte differentiating into foam cells induced by ox-LDL,whieh may be the action mechanism of AT in anti-atherosclerosis and in inhibiting inflammatory factor.