慢性丙型肝炎患者病毒基因型与IL-28B基因型分布

Distribution of the genotypes of hepatitis C virus and host interleukin 28B polymorphism in patients with chronic hepatitis C

目的了解慢性丙型肝炎患者白细胞介素-28B(IL-28B)基因型多态性分布的特点及其临床意义。方法在27例慢性丙型肝炎患者,分离外周血细胞DNA,采用IPLEX Gold法检测宿主IL-28B基因多态性;分析患者IL-28B基因型与血清丙型肝炎病毒(HCV)基因型、HCV RNA载量和肝功能指标的相关性。结果在27例慢性丙型肝炎患者中,感染HCV基因1型1例(3.7%),1b基因型7例(25.9%),其它基因型19例(19/27,70.4%);在IL-28B基因型中,rs12979860 CC基因型、rs12980275 AA基因型及rs8099917 TT基因型共24例(88.9%),而IL28B rs12979860 CT基因型、rs12980275 GA基因型和rs8099917 GT基因型共3例(11.1%);在HCV基因1型或1b型感染者中,IL28B rs12979860 CC基因型、rs12980275 AA基因型和rs8099917 TT基因型占62.5%(5/8),而HCV其他基因型感染者IL28B rs12979860 CC基因型、rs12980275 AA基因型和rs8099917 TT基因型占100%(19/19);HCV基因1型或1b型感染者与HCV其他基因型感染者比,其IL28B rs12979860位点、rs12980275位点和rs8099917位点基因型分布有显著性差异(P<0.01);IL-28B基因多态性分布与患者血清HCV RNA载量或肝功能指标的变化无显著性相关。结论本组慢性丙型肝炎患者HCV基因型大多为非1型;大多数感染者IL-28B基因为rs12979860 CC、rs12980275 AA和rs8099917 TT基因型。

Objective To investigate the distribution of the genotypes of hepatitis C virus （HCV）and host interleukin 28B polymorphism in patients with chronic hepatitis C. Methods A total of 27 patients were recruit-ed in this study and their blood samples were analyzed for HCV genotypes,viral loads,IL-28B genotypes and liv-er function index. Results There were eight out of twenty-seven （29.6%）patients infected with HCV genotype 1 or 1b, and other 19 （70.4%）with genotypes 2,3 or 6;The IL-28B CC rs12979860,AA rs12980275 and TT rs8099917 were present in 88.9%（24/27）of the patients and IL-28B CT rs12979860,GA rs12980275 or GT rs8099917 genotypes in 11.1%（3/27）of the patients;In patients infected with HCV genotype 1 or 1b,the IL-28B genotype rs12979860 CC,rs12980275 AA and rs8099917 TT accounted for 62.5%（5/8）,while in other patients, the IL-28B genotypes rs12979860 CC,rs12980275 AA,and rs8099917 TT accounted for 100%（19/19）;The IL-28B genotypes rs12979860,rs12990275 and rs8099917 in patients infected with HCV genotype 1 or 1b differed significantly from patients with other HCV genotypes （P＆lt;0.01）;However,no significant correlation was found be-tween the polymorphism of IL-28B genotypes and viral load or liver function index. Conclusion A majority of patients with chronic hepatitis C are infected with HCV genotype 2,3 or 6 and most of the patients carry IL-28B genotype rs12979860 CC,rs12980275 AA and rs8099917 TT.