复合酶制剂对黄颡鱼生长性能、血清生化和免疫指标的影响

Effects of complete enzyme preparation on growth performance,serum biochemical and immune indices of juvenile yellow catfish (Peltobagrus fulvidraco)

选取360尾初始体质量约为2.48 g的黄颡鱼（Pelteobagrus fulvidraco）幼鱼,随机分为4组,分别投喂添加0（对照）、100 mg·kg-1、200 mg·kg-1和400 mg·kg-1复合酶制剂的试验饲料,养殖8周。结果表明,黄颡鱼的增重率、特定生长率和摄食量随复合酶制剂添加量的增加而升高,其中400 mg·kg-1组的增重率、特定生长率和摄食量分别比对照组高出15.4%、7.5%和20.3%（P〈0.05）。各组黄颡鱼的粗蛋白质、粗脂肪、水分和灰分质量分数差异不显著（P〉0.05）。随着复合酶制剂水平的增加,血清尿素浓度逐渐降低,血清谷丙转氨酶、谷草转氨酶活力和总蛋白质量浓度升高,血清溶菌酶活力则先升高后降低,在100~200 mg·kg-1时有最高值（P〈0.05）;血清超氧化物歧化酶活力和丙二醛质量摩尔浓度均无显著性差异（P〉0.05）。饲料中添加400 mg·kg-1复合酶制剂能显著提高黄颡鱼的生长性能和血清谷丙转氨酶活力,降低血清尿素水平;200 mg·kg-1能显著提高血清总蛋白质量浓度、谷草转氨酶和溶菌酶活力。

We randomly assigned 360 juvenile yellow catfish（Peltobagrus fulvidraco） with average body weight 2.48 g to 4 groups,and fed the control（G0） with basal diet,the other 3 groups（G100,G200 and G400） with basal diet supplemented with 100 mg·kg-1,200 mg·kg-1 and 400 mg·kg-1 complete enzyme preparation（CEP）,respectively.After feeding for 8 weeks,the weight gain rate（WGR）,specific growth rate（SGR） and feed intake（FI） increased with the CEP concentration.Besides,the WGR,SGR and FI of G400 were higher than those of the control by 15.4 %,7.5 % and 20.3 %（P0.05）,respectively.No significant difference was found among all the treatments in body moisture,crude protein,crude lipid and ash content（P 0.05）.Serum urea content decreased with increasing CEP in diets,while glutamic-pyruvic transaminase（ALT）,glutamic-oxalacetic transaminase（AST） activities and total protein（TP） content increased（P 0.05）.The activity of lysozyme（LSZ） increased and then decreased（P0.05）.In conclusion,the feed added with 400 mg·kg-1 CEP can promote the growth and ALT activities of yellow catfish,and decrease its urea content;the feed added with 200 mg·kg-1 CEP can significantly increase the TP content and LSZ and AST activities of the fish.