大理白族2型糖尿病与脂联素基因多态性相关性分析

Genetic association analysis of the adiponectin polymorphisms in type 2 diabetes of Bai nationality at Dali area

目的检测云南省大理白族脂联素基因启动子单核苷酸多态性(SNP)与2型糖尿病的相关性。方法采用聚合酶链反应直接测序法在300例无血缘关系的云南省大理白族人群(健康者120例,2型糖尿病患者180例)中检测SNPs+45、+276的基因型,分析以上2个多态性位点与2型糖尿病的相关性。结果 SNP+45位点在健康者和2型糖尿病患者具有明显差异,SNP+276在两组差异无统计学意义。连锁不平衡分析显示,健康组和病例组间SNPs+45、+276存在一定差异。结论大理白族人群脂联素基因上SNP+45G与2型糖尿病的发生存在关联;SNP+45G是2型糖尿病发生的一项危险因素。

Objective Adiponectin gene is associated with BMI,insulin sensitivity and diabetes. To detect the impact of adiponectin gene promoter single nucleotide polymorphism（SNP） on type Ⅱ diabetes in Bai nationality of Yunnan province. Methods We detected adiponectin gene ＋276G/T and ＋45T/G with PCR- direct sequencing in 300 cases of unrelated people which contain healthy control subjects（n= 120） and type 2 diabetic patients（n= 180） in Bai nationality of Yunnan province, then analyzed their relationship with type 2 diabetes. Results SNP＋ 45 （G/T） showed significant difference of allele frequencies between type 2 diabetic group and the control group（P = 0.013）. Not statistically significant difference was found at adiponectin gene polymorphisms ＋276G/T in this study. Linkage disequilibrium（LD） of ＋ 276G/T and ＋ 45T/G between cases and controls showed significant differences. Conclusion The present study provides evidence that SNP＋45G in exon 2 region of the adiponectin gene contributes to the de- velopment of type 2 diabetes in Bai nationality of Yunnan province.