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系统性红斑狼疮患者抗双链DNA抗体检测及其与免疫学指标的相关性 被引量:5

Detection of anti-double-stranded DNA antibody and its correlation with immunological parameters in patients with systemic lupus erythematosus
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摘要 目的探讨系统性红斑狼疮患者抗双链DNA(dsDNA)抗体与免疫学指标的相关性。方法收集77例系统性红斑狼疮患者血清,采用短膜虫间接免疫荧光法(CLIFT)及酶联免疫吸附测定(ELISA)检测血清抗dsDNA抗体、总补体、补体C3、补体C4水平。结果 77例系统性红斑狼疮患者50例抗dsDNA抗体阳性,27例抗dsDNA抗体阴性。抗dsDNA抗体阳性组和阴性组患者血清IgG、IgM、IgA水平的差异没有统计学意义(P>0.05),抗dsDNA抗体阳性组患者血清总补体、补体C3、补体C4水平明显低于阴性患者(P<0.05)。抗dsDNA抗体阳性患者血清抗dsDNA抗体水平与总补体、补体C3显著相关(P<0.05)。结论抗dsDNA抗体可能通过补体旁路途径而非经典途径抑制补体生成。 Objective To investigate anti-double-stranded DNA(dsDNA)antibodiy and its correlation with immunological pa- rameters in patients with systemic lupus erythematosus.Methods 77 patients with systemic lupus erythematosus were enrolled. Crithidia luciliae immunofluorescence test(CLIFT)and enzyme-linked immunosorbent assay(ELISA)were used to detect serum levels of anti-dsDNA antibodies,total complement,complement C3 and complement C4.Results Among 77 patients with systemic lupus erythematosus,50 were found anti-dsDNA antibody positive,27 were found anti-dsDNA antibody negative.The differences of serum IgG,IgM,IgA levels between anti-dsDNA antibody positive and negative group were not statistically significant(P〈0.05). The serum levels of total complement,complement C3,complement C4 of patients with positive anti-dsDNA antibody were marked- ly lower than those of patients with negative anti-dsDNA antibody(P〈0.05).Serum level of anti-dsDNA antibody of patients with positive anti-dsDNA antibody demonstrated significant correlation with serum levels of total complement and complement C3(P〈0.05).Conclusion The anti-dsDNA antibodies may inhibit complement production via complement alternative pathway rather than classical pathway.
出处 《国际检验医学杂志》 CAS 2013年第23期3114-3115,3118,共3页 International Journal of Laboratory Medicine
基金 江西省教育厅青年科学基金(GJJ12625)
关键词 补体C3 补体C4 红斑狼疮 系统性 抗双链DNA抗体 complement C3 complement C4 lupus erythematosus systemic anti-double-stranded DNA
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  • 1Bombardier C, Gladman DD, Urowitz MB, et al. Derivation of the SLE: a disease activity index for lupus patients. Arthritis Rheum, 1992, 35: 630-640.
  • 2Arnett FC, Edworthy SM, Bloch DA, et al. The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum, 1988, 31:315-324.
  • 3Lopez-Hoyos M, Cabeza R. Clinical disease activity and titers of anti-dsDNA antibodies measured by an automated immunoiluorescence assay in patients with system/c lupus erythematosus. Lupus, 2005, 14:505-509.

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