9项呼吸道病原体检测在呼吸道感染病因分析中的应用

Application of nine respiratory pathogens detection in etiologic analysis of respiratory infection

目的探讨呼吸道感染的常见病因,为临床提供早期诊断依据。方法选择呼吸道感染患者3 514例,空腹采集静脉血,采用间接免疫荧光法进行呼吸道嗜肺军团菌1型(LP1)、肺炎支原体(MP)、Q热立克次体(COX)、肺炎衣原体(CP)、腺病毒(ADV)、呼吸道合胞病毒(RSV)、甲型流感病毒(IFA)、乙型流感病毒(IFB)、副流感病毒(PIV)9项病原体的联合检测。结果 3 514例患者血清IgM总阳性率为35.00%(1 230/3 514),IFB阳性率最高,为26.01%;其次为MP,阳性率为16.28%;PIV、ADV、RSV阳性率分别为0.03%、2.27%、0.65%。病原体合并感染413例,阳性率为11.75%(413/3 514)。结论呼吸道感染的病原体主要为病毒和MP。

Objective To investigate the common causes of respiratory infection,and provide basis for clinical early diagnosis. Methods 3 514 patients with respiratory tract infection were enrolled,and their fasting serum samples were collected.Indirect im- munofluorescence was employed to conduct joint detection for Legionella pneumophila 1（LP1）,Mycoplasma pneumoniae（MP）, Coxiella burnetii（COX）,Chlamydia pneumoniae（CP）,adenovirus（ADV）,respiratory syncytial virus（RSV）,influenza A virus（IFA）,influenza B virus（IFB）,parainfluenza virus（PIV）in respiratory tract.Results Among 3 514 patients,IgM-positive rate was 35.00%（1 230/3 514）.IFB showed the highest positive rate,26.01%,followed by MP,with positive rate of 16.28%,and PIV-, ADV-,RSV-positive rate were 0.03%,2.27%,0.65%,respectively.413 cases were found pathogens infection,with positive rate of 11.75%（413/3 514）.Conclusion Virus and MP are the main respiratory tract infection pathogens.