棉花DELLA蛋白GhGAI4a基因在拟南芥中功能初步分析

Functional Analysis of Cotton DELLA Protein GhGAI4a Gene in Arabidopsis thaliana

为了探究棉花DELLA蛋白基因GhGAI4a在拟南芥中的功能,构建植物表达载体pBP35S∶GhGAI4a和pBP35S∶Ghgai4a,利用农杆菌介导的花滴法将其转入Col野生型拟南芥。结果显示,2个载体各自获得6个独立的转基因拟南芥纯合株系。分别统计48-96h种子萌发率,测量生长7d后幼苗的主根长度,与非转基因植株相比,过量表达GhGAI4a、Ghgai4a对拟南芥种子萌发及主根生长具有明显的抑制作用。1μmol/L GA处理转基因植株,GhGAI4a转基因植株主根长和萌发率均增大,而Ghgai4a转基因植株主根长度和萌发率均无显著变化。

To analyze the function of cotton DELLA protein GhGAI4a gene in Arabidopsis thaliana, we constructed the plant expression vector pBP35S ： GhGAI4a and pBP35S ： Ghgai4a and converted them into Arabidopsis thaliana Col with the method of Agrobacterium-mediated inflorescence infection. Then through the kanamycin screening, the test of polymerase chain reaction （PCR） technology and the semi-quantitative RT-PCR analysis, we obtained six separate transgenic Arabidopsis homozygous from each of the two vectors. We made statistics about the 48-96 h seed germination rate,and measured the taproot length of the 7 d seedling. Compared with non-transgenetic plants,GhGAI4a and Ghgai4a have obvious inhibitory effect on seed germination and root growth. Meanwhile, through 1 μmol/L GA treatment, the taproot length and germination rate of GhGAI4a transgenic plants increased while the Ghgai4a transgenic plants did not change obviously.